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oai:repisalud.isciii.es:20.500.12105/254152025-12-18T13:01:52Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597

00925njm 22002777a 4500 dc Ndoye, Abibatou author Budina-Kolomets, Anna author Kugel, Curtis H author Webster, Marie R author Kaur, Amanpreet author Behera, Reeti author Rebecca, Vito W author Li, Ling author Brafford, Patricia A author Liu, Qin author Gopal, Y N Vashisht author Davies, Michael A author Mills, Gordon B author Xu, Xiaowei author Wu, Hong author Herlyn, Meenhard author Nicastri, Michael C author Winkler, Jeffrey D author Soengas, MS author Amaravadi, Ravi K author Murphy, Maureen E author Weeraratna, Ashani T author 2017-11-01 Autophagy mediates resistance to various anticancer agents. In melanoma, resistance to targeted therapy has been linked to expression of Wnt5A, an intrinsic inhibitor of β-catenin, which also promotes invasion. In this study, we assessed the interplay between Wnt5A and autophagy by combining expression studies in human clinical biopsies with functional analyses in cell lines and mouse models. Melanoma cells with high Wnt5A and low β-catenin displayed increased basal autophagy. Genetic blockade of autophagy revealed an unexpected feedback loop whereby knocking down the autophagy factor ATG5 in Wnt5A cells decreased Wnt5A and increased β-catenin. To define the physiologic relevance of this loop, melanoma cells with different Wnt status were treated and with the potent lysosomotropic compound Lys05. Wnt5A cells were less sensitive to Lys05 and could be reverted by inducing β-catenin activity. Our results suggest the efficacy of autophagy inhibitors might be improved by taking the Wnt signature of melanoma cells into account. . Cancer Res . 2017 Nov 1;77(21):5873-5885. Cancer Research https://pmc.ncbi.nlm.nih.gov/articles/PMC5718045/ 28887323 https://hdl.handle.net/20.500.12105/25415 BETA-CATENIN RESISTANCE THERAPY ADAPTER CELLS DEGRADATION PROGRESSION ACTIVATION SENESCENCE EXPRESSION ATG5 Mediates a Positive Feedback Loop between Wnt Signaling and Autophagy in Melanoma.