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oai:repisalud.isciii.es:20.500.12105/253742025-12-18T12:56:27Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19608col_20.500.12105_19609col_20.500.12105_16963col_20.500.12105_16978

00925njm 22002777a 4500 dc Brochado-Kith, Oscar author Rava, Marta author Berenguer, Juan author González-García, Juan author Rojo, David author Díez, Cristina author Hontañón, Víctor author Virseda-Berdices, Ana author Ibañez-Samaniego, Luis author Llop-Herrera, Elba author Olveira, Antonio author Pérez-Latorre, Leire author Barbas, Coral author Fernandez-Rodriguez, Amanda author Resino, Salvador author Jimenez-Sousa, Maria Angeles author ESCORIAL Study Group author 2024-10 Background: Altered bacterial translocation is associated with changes in hepatic function and the progression from compensated to decompensated cirrhosis. Child-Turcotte-Pugh (CTP) score is an essential indicator of liver severity. Thus, we aimed to study differences in the blood microbiome together with metabolome profile between HCV-infected patients with CTP class B (CTP-B, significant functional compromise) and patients with CTP class A (CTP-A, well-compensated cirrhosis). Methods: We conducted a cross-sectional study in patients with advanced HCV-related cirrhosis (n = 88) stratified by CTP-B and CTP-A. Bacterial 16S rRNA sequencing was sequenced by MiSeq Illumina technology and non-targeted metabolomics was performed by GC-MS and LC-MS ESI+ and ESI- to complement the analysis. Results: Patients with CTP-B had lower levels of richness (Chao1), and alpha diversity (Shannon and Simpson indexes) at phylum level than patients with CTP-A. Likewise, we observed significant differences in beta diversity between groups at phylum, class, and order levels, showing lower diversity in patients with CTP-B. Higher relative abundance of Proteobacteria (p = 0.012), Alphaproteobacteria (p = 0.005), Sphingomonadales (p = 0.012) and Sphingomonadaceae (p = 0.016) were significantly associated with CTP-B. The phylum Proteobacteria was positively correlated with ethanolamine and oleic acid (p = 0.005 and p = 0.004, respectively) and negatively with p-cresol (p = 0.006). In addition, the order Sphingomonadales and the family Sphingomonadaceae was also negatively correlated with p-cresol (p = 0.001 and p = 0.001). Conclusions: Blood microbial diversity was significantly decreased in patients with CTP-B, who presented an enrichment of Proteobacteria, Alphaproteobacteria, Sphingomonadales and Sphingomonadaceae compared to patients with CTP-A. J Infect Public Health. 2024 Oct;17(10):102524. 1876-0341 https://hdl.handle.net/20.500.12105/25374 39241484 10.1016/j.jiph.2024.102524 1876-035X Journal of infection and public health Child-Pugh Chronic hepatitis C Cirrhosis HIV Metabolome Microbiome Altered blood microbiome in patients with HCV-related Child-Pugh class B cirrhosis