2026-06-14T03:45:16Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/253712025-12-18T12:59:33Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617col_20.500.12105_25179
Repisalud
author
Mateos-Martínez, Patricia
author
Coronel Lopez, Raquel
author
Sachse, Martin
author
González-Sastre, Rosa
author
Maeso Cuesta, Laura
author
Rodríguez, María Josefa
author
Terrón-Orellana, Maria Carmen
author
López-Alonso, Victoria
author
Liste-Noya, Isabel
funder
Ministerio de Ciencia e Innovación (España)
funder
Agencia Estatal de Investigación (España)
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
2024-10-29T13:27:00Z
2024-10-29T13:27:00Z
2024
Front Cell Neurosci. 2024 Jun 12:18:1406839.
10.3389/fncel.2024.1406839
1662-5102
Frontiers in cellular neuroscience
38933177
https://hdl.handle.net/20.500.12105/25371
Introduction: Human cerebral organoids (hCOs) derived from pluripotent stem cells are very promising for the study of neurodevelopment and the investigation of the healthy or diseased brain. To help establish hCOs as a powerful research model, it is essential to perform the morphological characterization of their cellular components in depth. Methods: In this study, we analyzed the cell types consisting of hCOs after culturing for 45 days using immunofluorescence and reverse transcriptase qualitative polymerase chain reaction (RT-qPCR) assays. We also analyzed their subcellular morphological characteristics by transmission electron microscopy (TEM). Results: Our results show the development of proliferative zones to be remarkably similar to those found in human brain development with cells having a polarized structure surrounding a central cavity with tight junctions and cilia. In addition, we describe the presence of immature and mature migrating neurons, astrocytes, oligodendrocyte precursor cells, and microglia-like cells. Discussion: The ultrastructural characterization presented in this study provides valuable information on the structural development and morphology of the hCO, and this information is of general interest for future research on the mechanisms that alter the cell structure or function of hCOs.
eng
Glial cells
Human brain organoids
Human pluripotent stem cells
Mini-brains
Neural stem cells
Neurodevelopment
Transmission electron microscopy
Ultrastructural characterization
Human cerebral organoids: cellular composition and subcellular morphological features
research article
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URL
https://repisalud.isciii.es/bitstreams/a3bd9e01-3599-4bf6-9b43-f4b413918f58/download
File
MD5
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application/pdf
HumanCerebralOrganoidsCellularComposition_2024.pdf
URL
https://repisalud.isciii.es/bitstreams/353bb1a9-53b3-44d1-9b49-3ee5dc0cb9c7/download
File
MD5
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text/plain
HumanCerebralOrganoidsCellularComposition_2024.pdf.txt