2026-06-14T03:52:25Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/253712025-12-18T12:59:33Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617col_20.500.12105_25179

00925njm 22002777a 4500 dc Mateos-Martínez, Patricia author Coronel Lopez, Raquel author Sachse, Martin author González-Sastre, Rosa author Maeso Cuesta, Laura author Rodríguez, María Josefa author Terrón-Orellana, Maria Carmen author López-Alonso, Victoria author Liste-Noya, Isabel author 2024 Introduction: Human cerebral organoids (hCOs) derived from pluripotent stem cells are very promising for the study of neurodevelopment and the investigation of the healthy or diseased brain. To help establish hCOs as a powerful research model, it is essential to perform the morphological characterization of their cellular components in depth. Methods: In this study, we analyzed the cell types consisting of hCOs after culturing for 45 days using immunofluorescence and reverse transcriptase qualitative polymerase chain reaction (RT-qPCR) assays. We also analyzed their subcellular morphological characteristics by transmission electron microscopy (TEM). Results: Our results show the development of proliferative zones to be remarkably similar to those found in human brain development with cells having a polarized structure surrounding a central cavity with tight junctions and cilia. In addition, we describe the presence of immature and mature migrating neurons, astrocytes, oligodendrocyte precursor cells, and microglia-like cells. Discussion: The ultrastructural characterization presented in this study provides valuable information on the structural development and morphology of the hCO, and this information is of general interest for future research on the mechanisms that alter the cell structure or function of hCOs. Front Cell Neurosci. 2024 Jun 12:18:1406839. 10.3389/fncel.2024.1406839 1662-5102 Frontiers in cellular neuroscience 38933177 https://hdl.handle.net/20.500.12105/25371 Glial cells Human brain organoids Human pluripotent stem cells Mini-brains Neural stem cells Neurodevelopment Transmission electron microscopy Ultrastructural characterization Human cerebral organoids: cellular composition and subcellular morphological features