2026-04-29T12:24:22Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/253592025-12-18T13:01:02Zcom_20.500.12105_2273com_20.500.12105_2202col_20.500.12105_19571
Repisalud
author
Moreno-Oñate, M
author
Herrero-Ruiz, A M
author
García-Dominguez, M
author
Cortes-Ledesma, Felipe
author
Ruiz, J F
funder
Ministerio de Economía y Competitividad (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Unión Europea. Comisión Europea. European Research Council (ERC)
funder
Universidad de Sevilla (España)
2024-10-29T09:01:56Z
2024-10-29T09:01:56Z
2020-06-12
J Mol Biol . 2020 Jun 12;432(13):3965-3979.
Journal of Molecular Biology
32224012
https://hdl.handle.net/20.500.12105/25359
Cellular DNA is under constant attack by a wide variety of agents, both endogenous and exogenous. To counteract DNA damage, human cells have a large collection of DNA repair factors. Among them, DNA polymerase lambda (Polλ) stands out for its versatility, as it participates in different DNA repair and damage tolerance pathways in which gap-filling DNA synthesis is required. In this work, we show that human Polλ is conjugated with Small Ubiquitin-like MOdifier (SUMO) proteins both in vitro and in vivo, with Lys27 being the main target of this covalent modification. Polλ SUMOylation takes place in the nuclear pore complex and is mediated by the E3 ligase RanBP2. This post-translational modification promotes Polλ entry into the nucleus, which is required for its recruitment to DNA lesions and stimulated by DNA damage induction. Our work represents an advance in the knowledge of molecular pathways that regulate cellular localization of human Polλ, which are essential to be able to perform its functions during repair of nuclear DNA, and that might constitute an important point for the modulation of its activity in human cells.
eng
DNA polymerase lambda
DNA repair
SUMOylation
post-translational modifications
RanBP2-Mediated SUMOylation Promotes Human DNA Polymerase Lambda Nuclear Localization and DNA Repair.
research article
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URL
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RanBP2-MediatedSUMOylation-2020.pdf
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https://repisalud.isciii.es/bitstreams/cae89562-a38f-449e-b5a3-f58c6749b1c2/download
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RanBP2-MediatedSUMOylation-2020.pdf.txt