2026-05-01T03:22:07Zhttps://repisalud.isciii.es/rest/oai/request
oai:repisalud.isciii.es:20.500.12105/252782024-10-23T13:07:23Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16958
Repisalud author Olmo, Alberto author Yuste, Yaiza author Serrano, Juan Alfonso author Maldonado-Jacobi, Andres author Pérez, Pablo author Huertas, Gloria author Pereira, Sheila author Yufera, Alberto author de la Portilla, Fernando 2024-10-23T13:07:23Z 2024-10-23T13:07:23Z 2020-06-02 10.3390/s20113152 1424-8220 Sensors (Basel, Switzerland) http://hdl.handle.net/10668/15681 32498394 https://hdl.handle.net/20.500.12105/25278 In tissue engineering, of utmost importance is the control of tissue formation, in order to form tissue constructs of clinical relevance. In this work, we present the use of an impedance spectroscopy technique for the real-time measurement of the dielectric properties of skeletal myoblast cell cultures. The processes involved in the growth and differentiation of these cell cultures in skeletal muscle are studied. A circuit based on the oscillation-based test technique was used, avoiding the use of high-performance circuitry or external input signals. The effect of electrical pulse stimulation applied to cell cultures was also studied. The technique proved useful for monitoring in real-time the processes of cell growth and estimating the fill factor of muscular stem cells. Impedance spectroscopy was also useful to study the real-time monitoring of cell differentiation, obtaining different oscillation amplitude levels for differentiated and undifferentiated cell cultures. Finally, an electrical model was implemented to better understand the physical properties of the cell culture and control the tissue formation process. eng Electrical modeling Impedance spectroscopy Oscillation-based test Skeletal muscle Stem cell differentiation Electrical Modeling of the Growth and Differentiation of Skeletal Myoblasts Cell Cultures for Tissue Engineering. research article