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00925njm 22002777a 4500 dc Vargas, S L author Ponce, C author Bustamante, R author Calderón, E author Nevez, G author De Armas, Y author Matos, O author Miller, R F author Gallo, M J author 2017-06-05 To understand the epidemiological significance of Pneumocystis detection in a lung tissue sample of non-immunosuppressed individuals, we examined sampling procedures, laboratory methodology, and patient characteristics of autopsy series reported in the literature. Number of tissue specimens, DNA-extraction procedures, age and underlying diagnosis highly influence yield and are critical to understand yield differences of Pneumocystis among reports of pulmonary colonization in immunocompetent individuals. 10.1007/s10096-017-3006-8 1435-4373 European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology http://hdl.handle.net/10668/11275 28584896 https://hdl.handle.net/20.500.12105/25222 Importance of tissue sampling, laboratory methods, and patient characteristics for detection of Pneumocystis in autopsied lungs of non-immunosuppressed individuals.