2026-05-04T19:37:41Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/251912025-12-18T12:56:51Zcom_20.500.12105_15322com_20.500.12105_2051com_20.500.12105_2052col_20.500.12105_16963col_20.500.12105_16966col_20.500.12105_25179

00925njm 22002777a 4500 dc Casado-Fernández, Laura author Laso-García, Fernando author Piniella, Dolores author Gómez-de Frutos, Mari Carmen author Otero-Ortega, Laura author Bravo, Susana-Belén author Fuentes-Gimeno, Blanca author Docando, Felix author Alonso-López, Elisa author Ruiz-Ares, Gerardo author Rodríguez-Pardo, Jorge author Rigual, Ricardo author de Celis-Ruiz, Elena author Hervás, Carlos author Díez-Tejedor, Exuperio author Gutiérrez-Fernández, María author Alonso de Leciñana, Maria author 2024-10-15 Circulating extracellular vesicles (EVs) can participate in innate repair processes triggered after intracerebral hemorrhage (ICH). We aimed to describe changes in the proteomic profile of circulating EVs between the acute and subacute phases of ICH and to compare the findings depending on outcomes, as an approach to unraveling such repair mechanisms. This was a prospective observational study including patients with non-traumatic supratentorial ICH. Exclusion criteria were previous disability, signs of herniation on baseline computed tomography, or limited life expectancy. EVs were isolated from blood samples at 24 h and 7 days after symptom onset. After 6-months' follow-up, patients were dichotomized into poor and good outcomes, defining good as an improvement of >10 points or > 50 % on the National Institutes of Health Stroke Scale and a modified Rankin Scale of 0-2. The protein cargo was analyzed by quantitative mass spectrometry and compared according to outcomes. Forty-four patients completed follow-up, 16 (35.5 %) having good outcomes. We identified 1321 proteins in EVs, 37 with differential abundance. In patients with good outcomes, proteins related to stress response (DERA, VNN2, TOMM34) and angiogenesis (RHG01) had increased abundance at 7 days. EVs from patients with poor outcomes showed higher levels of acute-phase reactants (CRP, SAA2) at 7 days compared with 24 h. In conclusion, the protein content of circulating EVs in patients with ICH changes over time, the changes varying depending on the clinical outcome, with greater abundance of proteins potentially involved in the repair processes of patients with good outcomes. Neurobiol Dis. 2024 Oct 15:201:106665. 10.1016/j.nbd.2024.106665 1095-953X 0969-9961 Neurobiology of disease 39277144 https://hdl.handle.net/20.500.12105/25191 Extracellular vesicles Intracerebral hemorrhage Pathophysiology Proteomics The proteomic signature of circulating extracellular vesicles following intracerebral hemorrhage: Novel insights into mechanisms underlying recovery