2026-04-29T04:17:38Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/251782025-12-18T12:59:08Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617
Repisalud
author
Tejerina-Miranda, Sandra
author
Gamella, Maria
author
Pedrero, María
author
Montero-Calle, Ana Maria
author
Rejas-González, Raquel
author
Pingarrón, José M
author
Barderas Manchado, Rodrigo
author
Campuzano, Susana
funder
Agencia Estatal de Investigación (España)
funder
Unión Europea. Comisión Europea. H2020
funder
Ministerio de Ciencia e Innovación (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Instituto de Salud Carlos III
funder
Complutense University of Madrid (España)
2024-10-17T10:27:20Z
2024-10-17T10:27:20Z
2024
Electrochimica Acta. 2024, 503:144822.
0013-4686
https://hdl.handle.net/20.500.12105/25178
10.1016/j.electacta.2024.144822
Electrochimica Acta
In this work, we report the development of electrochemical bioplatforms for both the single determination of TIMP-1 and the simultaneous determination of TIMP-1 and GDF-15, two biomarkers of cellular senescence and prognosis in colorectal cancer (CRC). The designed immunoplatforms rely on a sandwich-type configuration labeled with horseradish peroxidase (HRP) built on magnetic microsupports and involve a pair of specific an tibodies to capture and detect each target protein. Amperometric readout was performed upon trapping the magnetic bioconjugates on the surface of either single or dual disposable carbon electrodes, using the hydro quinone/hydrogen peroxide (HQ/H2O2) system. The attractive analytical performance of the TIMP-1 bio platform, achieving a LOD of 13.0 pg mL‒1 and a dynamic range between 43.4 and 2500 pg mL‒1, led us to exploit it for TIMP-1 determination in cell extracts and tissues of CRC samples. The results showed the possibility of discriminating the metastatic capabilities of cells and detecting CRC patients, after just a simple dilution and in only 60 min. The multiplexing feasibility of the developed immunoplatform allowed the implementation of a dual assay for the simultaneous determination of TIMP-1 (LOD 19.2 pg mL‒1) and GDF-15 (LOD 16.6 pg mL‒1 ). The dual platform was used for the analysis of CRC samples (plasma and cell secretomes) in just 75 min and using a very simple protocol. These characteristics make the developed immunoplatform a very attractive tool to face particularly challenging samples, such as secretomes or plasma from CRC patients, due to the required sensi tivity, thus providing a better snapshot of CRC.
eng
Electrochemical immunoplatforms
Dual determination
Colorectal cancer
Cellular senescence
Plasma
Tissues
Dual immunoplatform to assess senescence biomarkers TIMP-1 and GDF-15: Advancing in the understanding of colorectal cancer
research article
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
URL
https://repisalud.isciii.es/bitstreams/a9928494-e5aa-4a1f-b96f-0fdea40a767f/download
File
MD5
cc8f2cc2f615e51180dbff586e0b67cd
3920585
application/pdf
DualImmunoplatformAssessSenescence_2024.pdf
URL
https://repisalud.isciii.es/bitstreams/1e65cf89-b10a-4129-9f9d-412856a70392/download
File
MD5
c32916bc7a618c05c189fcd31cab2086
510469
application/pdf
Supplementary_DualImmunoplatformAssessSenescence_2024.pdf
URL
https://repisalud.isciii.es/bitstreams/8f75b998-280e-4be1-9df8-55ad1caa4a93/download
File
MD5
ffdaf2515169bec0631c3519d01c8f78
80883
text/plain
DualImmunoplatformAssessSenescence_2024.pdf.txt
URL
https://repisalud.isciii.es/bitstreams/470160d4-2f86-4c7e-9023-8a78e6993e5c/download
File
MD5
ace070ac33c12b1c48ac89d384460e20
10142
text/plain
Supplementary_DualImmunoplatformAssessSenescence_2024.pdf.txt