2026-07-21T09:04:43Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/250982025-12-18T12:55:45Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617
Repisalud
author
Rejas-González, Raquel
author
Montero-Calle, Ana Maria
author
Pastora Salvador, Natalia
author
Crespo Carballés, María José
author
Ausín-González, Emma
author
Sánchez-Naves, Juan
author
Pardo Calderón, Sara
author
Barderas Manchado, Rodrigo
author
Guzman-Aranguez, Ana
funder
Banco Santander
funder
Complutense University of Madrid (España)
funder
Agencia Estatal de Investigación (España)
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
2024-10-14T11:25:53Z
2024-10-14T11:25:53Z
2024-09-21
Redox Biol. 2024 Sep 21:77:103368.
10.1016/j.redox.2024.103368
2213-2317
Redox biology
39326071
https://hdl.handle.net/20.500.12105/25098
Chronic ocular pathologies such as cataracts and glaucoma are emerging as an important problem for public health due to the changes in lifestyle and longevity. These age-related ocular diseases are largely mediated by oxidative stress. Small extracellular vesicles (sEVs) are involved in cell-to-cell communication and transport. There is an increasing interest about the function of small extracellular vesicles (sEVs) in the eye. However, the proteome content and characterization of sEVs released by ocular cells under pathological conditions are not yet well known. Here, we aimed to analyze the protein profile of sEVs and the intracellular protein content from two ocular cell lines (lens epithelial cells and retinal ganglion cells) exposed to oxidative stress to identify altered proteins that could serve as potential diagnostic biomarkers. The protein content was analyzed by quantitative mass spectrometry-based proteomics. Validation was performed by WB and ELISA using cell extracts and aqueous humor from cataract and glaucoma patients. After data analysis, 176 and 7 dysregulated proteins with an expression ratio≥1.5 were identified in lens epithelial cells' protein extract and sEVs, respectively, upon oxidative stress induction. In retinal ganglion cells, oxidative stress induction resulted in the dysregulation of 1033 proteins in cell extracts and 9 proteins in sEVs. In addition, by WB and ELISA, the dysregulation of proteins was mostly confirmed in aqueous humor samples from cataract or glaucoma patients in comparison to ICL individuals, with RAD23B showing high glaucoma diagnostic ability. Importantly, this work expands the knowledge of the proteome characterization of cataracts and glaucoma and provides new potential diagnostic glaucoma biomarkers.
eng
Aqueous humor
Cataracts
Glaucoma
Glaucoma diagnosis
Proteomics
Small extracellular vesicles
Unraveling the nexus of oxidative stress, ocular diseases, and small extracellular vesicles to identify novel glaucoma biomarkers through in-depth proteomics
research article
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