2026-07-24T07:37:24Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/238422024-11-28T20:12:41Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967

00925njm 22002777a 4500 dc Teixeira, Simao PB author Pardo, Alberto author Bakht, Syeda M author Gomez-Florit, Manuel author Reis, Rui L author Gomes, Manuela E author Domingues, Rui MA author 2024-02-16 Tendon injuries and tendinopathies are increasingly prevalent health problems currently lacking effective treatments. Tissue engineering offers promising strategies to boost the low innate regenerative ability of tendons. Within this context, the simultaneous leveraging of both physical and biochemical cues by engineered scaffolding systems can be explored to promote a stronger tenogenic response from stem cells. Here, molecularly imprinted polymeric nanoparticles (MINPs) against transforming growth factor (TGF)-β3 are combined with bioinspired anisotropic hydrogels to produce tenogenesis-inductive constructs. MINPs are first solid phase-imprinted against a TGF-β3 epitope, achieving an affinity comparable to monoclonal antibodies. MINPs and magnetically-responsive microfibers are then encapsulated together with adipose-derived stem cells within gelatin-based hydrogels, applying a magnetostatic field during gelation to align the microfibers. The created anisotropic microstructure guides cell growth and elongation unidirectionally, while MINPs act as artificial receptors for TGF-β3, potentiating its paracrine action in the cellular microenvironment. The combination of both stimuli proves effective at increasing TGF-β signaling, which promotes the expression of tendon-associated genes and corresponding protein synthesis, suggesting that microstructural cues and biomolecule sequestration act in tandem to direct cell fate commitment. Overall, this system recapitulates several elements of tendon development, constituting a promising strategy for the regeneration of this tissue. Teixeira SPB, Pardo A, Bakht SM, Gomez-Florit M, Reis RL, Gomes ME, et al. Guiding Stem Cell Tenogenesis by Modulation of Growth Factor Signaling and Cell-Scale Biophysical Cues in Bioengineered Constructs. Adv Funct Mater. 2024 Feb 16. 10.1002/adfm.202312961 Advanced Functional Materials https://hdl.handle.net/20.500.13003/20227 2-s2.0-85185106459 https://hdl.handle.net/20.500.12105/23842 1151954700001 Guiding Stem Cell Tenogenesis by Modulation of Growth Factor Signaling and Cell-Scale Biophysical Cues in Bioengineered Constructs