2026-04-29T20:47:14Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/237002024-11-28T23:00:02Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967

00925njm 22002777a 4500 dc Alorda-Clara, Marina author Reyes Moreno, Jose author Trelles-Guzman, Marita Grimanesa author Florido Garcia, Monica author Roca, Pilar author Pons, Daniel-Gabriel author Oliver, Jordi author 2023-04-17 Colorectal cancer (CRC) is the third most common cancer worldwide and is detected in late stages because of a lack of early and specific biomarkers. Tumors can release extracellular vesicles (EVs), which participate in different functions, such as carrying nucleic acids to target cells; promoting angiogenesis, invasion, and metastasis; and preparing an adequate tumor microenvironment. Finally, bowel lavage fluid (BLF) is a rarely used sample that is obtained during colonoscopy. It presents low variability and protein degradation, is easy to handle, and is representative of EVs from tumor cells due to proximity of the sample collection. This sample has potential as a research tool and possible biomarker source for CRC prognosis and monitoring. In this study, EVs were isolated from human BLF by ultracentrifugation, then characterized by transmission electron microscopy and atomic force microscopy. EV concentration was determined by nanoparticle tracking analysis, and tetraspanins were determined by Western blot, confirming correct EV isolation. RNA, DNA, and proteins were isolated from these EVs; RNA was used in real-time PCR, and proteins were used in an immunoblotting analysis, indicating that EV cargo is optimal for use and study. These results indicate that EVs from BLF can be a useful tool for CRC study and could be a source of biomarkers for the diagnosis and monitoring of CRC. Alorda-Clara M, Reyes J, Trelles-Guzman MG, Florido M, Roca P, Pons DG, et al. Isolation and Characterization of Extracellular Vesicles in Human Bowel Lavage Fluid. Int J Mol Sci. 2023 Apr 17;24(8). 10.3390/ijms24087391 1422-0067 International journal of molecular sciences https://hdl.handle.net/20.500.13003/18964 37108550 L2023002857 2-s2.0-85156093061 https://hdl.handle.net/20.500.12105/23700 979204300001 Isolation and Characterization of Extracellular Vesicles in Human Bowel Lavage Fluid