2026-04-29T07:22:51Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/236622024-11-29T07:15:51Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967
Repisalud
author
Banaee, Mahdi
author
Sagvand, Shiva
author
Sureda Gomila, Antoni
author
Amini, Mohammad
author
Haghi, Behzad Nematdoost
author
Sopjani, Mentor
author
Faggio, Caterina
2024-10-09T06:34:18Z
2024-10-09T06:34:18Z
2023-06
Banaee M, Sagvand S, Sureda A, Amini M, Haghi BN, Sopjani M, et al. Evaluation of single and combined effects of mancozeb and metalaxyl on the transcriptional and biochemical response of zebrafish (Danio rerio). Comp Biochem Physiol C Toxicol Pharmacol. 2023 Jun;268:109597.
1532-0456
https://hdl.handle.net/20.500.13003/20041
https://hdl.handle.net/20.500.12105/23662
36889533
10.1016/j.cbpc.2023.109597
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
2-s2.0-85150882621
1042591100001
L2023201056
Mancozeb and metalaxyl are fungicidal agents frequently used in combination to control fungi in crops that may affect non-target organisms when entering ecosystems. This study aims to evaluate the environmental effects of Mancozeb (MAN) and Metalaxyl (MET), alone and in combination, on zebrafish (Danio rerio) as an experimental model. The oxidative stress biomarkers and the transcription of genes involved in detoxification in zebrafish (Danio rerio) were assessed after co-exposure to MAN (0, 5.5, and 11 μg L-1) and MET (0, 6.5, and 13 mg L-1) for 21 days. Exposure to MAN and MET induced a significant increase in the expression of genes related to detoxification mechanisms (Ces2, Cyp1a, and Mt2). Although Mt1 gene expression increased in fish exposed to 11 μg L-1 of MAN combined with 13 mg L-1 of MET, Mt1 expression was down-regulated significantly in other experimental groups (p < 0.05). The combined exposure to both fungicides showed synergistic effects in the expression levels that are manifested mainly at the highest concentration. Although a significant (p < 0.05) increase in alkaline phosphatase (ALP) and transaminases (AST and ALT), catalase activities, the total antioxidant capacity, and malondialdehyde (MDA) contents in the hepatocytes of fish exposed to MAN and MET alone and in combination was detected, lactate dehydrogenase (LDH), gamma-glutamyl transferase (GGT) activities, and hepatic glycogen content decreased significantly (p < 0.05). Overall, these results emphasize that combined exposure to MET and MAN can synergistically affect the transcription of genes involved in detoxification (except Mt1 and Mt2) and biochemical indicators in zebrafish.
eng
Evaluation of single and combined effects of mancozeb and metalaxyl on the transcriptional and biochemical response of zebrafish (Danio rerio)
research article