2026-07-23T20:21:44Zhttps://repisalud.isciii.es/rest/oai/request
oai:repisalud.isciii.es:20.500.12105/236552024-10-09T06:34:12Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967
Repisalud author Gutiérrez-Capitán, Manuel author Sanchís, Ana author Carvalho, Estela O author Baldi, Antonio author Vilaplana, Lluïsa author Cardoso, Vanessa F author Calleja, Álvaro author Wei, Mingxing author de la Rica, Roberto author Hoyo, Javier author Bassegoda, Arnau author Tzanov, Tzanko author Marco, María-Pilar author Lanceros-Méndez, Senentxu author Fernández-Sánchez, César 2024-10-09T06:34:12Z 2024-10-09T06:34:12Z 2023-08-25 Gutiérrez-Capitán M, Sanchís A, Carvalho EO, Baldi A, Vilaplana L, Cardoso VF, et al. Engineering a Point-of-Care Paper-Microfluidic Electrochemical Device Applied to the Multiplexed Quantitative Detection of Biomarkers in Sputum. ACS Sensors. 2023 Aug 25;8(8):3032-42. 10.1021/acssensors.3c00523 2379-3694 ACS sensors https://hdl.handle.net/20.500.13003/19358 37467113 L641846244 2-s2.0-85166769666 https://hdl.handle.net/20.500.12105/23655 1032171200001 Health initiatives worldwide demand affordable point-of-care devices to aid in the reduction of morbidity and mortality rates of high-incidence infectious and noncommunicable diseases. However, the production of robust and reliable easy-to-use diagnostic platforms showing the ability to quantitatively measure several biomarkers in physiological fluids and that could in turn be decentralized to reach any relevant environment remains a challenge. Here, we show the particular combination of paper-microfluidic technology, electrochemical transduction, and magnetic nanoparticle-based immunoassay approaches to produce a unique, compact, and easily deployable multiplex device to simultaneously measure interleukin-8, tumor necrosis factor-α, and myeloperoxidase biomarkers in sputum, developed with the aim of facilitating the timely detection of acute exacerbations of chronic obstructive pulmonary disease. The device incorporates an on-chip electrochemical cell array and a multichannel paper component, engineered to be easily aligned into a polymeric cartridge and exchanged if necessary. Calibration curves at clinically relevant biomarker concentration ranges are produced in buffer and artificial sputum. The analysis of sputum samples of healthy individuals and acutely exacerbated patients produces statistically significant biomarker concentration differences between the two studied groups. The device can be mass-produced at a low cost, being an easily adaptable platform for measuring other disease-related target biomarkers. eng Engineering a Point-of-Care Paper-Microfluidic Electrochemical Device Applied to the Multiplexed Quantitative Detection of Biomarkers in Sputum research article