2026-06-14T00:40:41Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/236062024-11-28T21:41:57Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967

00925njm 22002777a 4500 dc Delgado, Jose Antonio author Pastor Garcia, Maria I author Márquez Jiménez, Nuria author Costa Petit, Gemma author Pablo Cànaves, Josep A author Robles, Juan author Bauça, Josep Miquel author 2023-02-15 Background-aim: High vitamin B12 concentrations are considered a common finding in clinical practice. Thanks to immunoassay accessibility, vitamin B12 has become a usual test in routine health checkups. However, these analytical methods usually present antibody-mediated interferences. Our aim was to propose an algorithm for the screening of antibody-mediated analytical interferences on vitamin B12 immunoassays on the Alinity platform. Methods: Observational, prospective, case-control study was performed during 12 months. Individuals with persistently elevated cobalamin concentrations [>554 pmol/L] were considered as cases in the absence of supplementation or other justifying cause. Individuals under treatment with vitamin B12, or in the context of alcoholism were included as controls. A thorough interference study by macromolecules in immunoassays was performed in serum samples: PEG precipitation, rheumatoid factor, heterophile antibodies and gel permeation chromatography (GPC). Albumin, total B12, IgG and IgM were measured in every GPC collected fraction and chromatograms were drafted. Results: Up to 45% of cases presented interference by B12-immunocomplexes and the precipitation for all of them was >50%. The individual with the lowest interfered vitamin B12 result was 661 pmol/L. Conclusion: The presence of antibody-mediated interferences, mainly B12-immunocomplexes, is a relatively common phenomenon. A simple algorithm for the screening of interferences is useful and reliable in ruling out healthy individuals and highly cost-effective. Delgado JA, Pastor García MI, Jiménez NM, Petit GC, Pablo Cánaves JA, Robles J, et al. Challenges in the diagnosis of hypervitaminemia B12. Interference by immunocomplexes. Clin Chim Acta. 2023 Feb;541:117267. 10.1016/j.cca.2023.117267 1873-3492 Clinica chimica acta: international journal of clinical chemistry https://hdl.handle.net/20.500.13003/19087 36805891 L2022940794 2-s2.0-85148663126 https://hdl.handle.net/20.500.12105/23606 965581800001 Challenges in the diagnosis of hypervitaminemia B12. Interference by immunocomplexes