2026-07-24T06:21:30Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/235692024-11-28T19:48:04Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967

00925njm 22002777a 4500 dc Llinàs-Arias, Pere author Ensenyat-Mendez, Miquel author Orozco, Javier IJ author Iñiguez-Muñoz, Sandra author Valdez, Betsy author Wang, Chuan author Mezger, Anja author Choi, Eunkyoung author Tran, Yan Zhou author Yao, Liqun author Bonath, Franziska author Olsen, Remi-André author Ormestad, Mattias author Esteller, Manel author Lupien, Mathieu author Marzese, Diego M author 2023-11-02 Objectives: Triple-negative breast cancer (TNBC) is a highly aggressive breast cancer subtype with limited treatment options. Unlike other breast cancer subtypes, the scarcity of specific therapies and greater frequencies of distant metastases contribute to its aggressiveness. We aimed to find epigenetic changes that aid in the understanding of the dissemination process of these cancers. Data description: Using CRISPR/Cas9, our experimental approach led us to identify and disrupt an insulator element, IE8, whose activity seemed relevant for cell invasion. The experiments were performed in two well-established TNBC cellular models, the MDA-MB-231 and the MDA-MB-436. To gain insights into the underlying molecular mechanisms of TNBC invasion ability, we generated and characterized high-resolution chromatin interaction (Hi-C) and chromatin accessibility (ATAC-seq) maps in both cell models and complemented these datasets with gene expression profiling (RNA-seq) in MDA-MB-231, the cell line that showed more significant changes in chromatin accessibility. Altogether, our data provide a comprehensive resource for understanding the spatial organization of the genome in TNBC cells, which may contribute to accelerating the discovery of TNBC-specific alterations triggering advances for this devastating disease. Llinàs-Arias P, Ensenyat-Méndez M, Orozco JIJ, Íñiguez-Muñoz S, Valdez B, Wang C, et al. 3-D chromatin conformation, accessibility, and gene expression profiling of triple-negative breast cancer. BMC Genomic Data. 2023 Nov 2;24(1):61. 10.1186/s12863-023-01166-x 2730-6844 BMC genomic data https://hdl.handle.net/20.500.13003/19967 37919672 L2026427195 2-s2.0-85175719906 https://hdl.handle.net/20.500.12105/23569 1092189200001 3-D chromatin conformation, accessibility, and gene expression profiling of triple-negative breast cancer