2026-05-17T00:18:28Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/235452024-11-28T19:42:24Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967
Repisalud
author
Graca, Ana L
author
Domingues, Rui MA
author
Calejo, Isabel
author
Gomez-Florit, Manuel
author
Gomes, Manuela E
2024-10-04T13:58:02Z
2024-10-04T13:58:02Z
2022-03
Graca AL, Domingues RMA, Calejo I, Gomez-Florit M, Gomes ME. Therapeutic Effects of Platelet-Derived Extracellular Vesicles in a Bioengineered Tendon Disease Model. Int J Mol Sci. 2022 Mar;23(6):2948.
10.3390/ijms23062948
1422-0067
International Journal of Molecular Sciences
https://hdl.handle.net/20.500.13003/19872
35328370
L2015890998
2-s2.0-85125998564
https://hdl.handle.net/20.500.12105/23545
775309600001
Tendon injuries represent over 30-50% of musculoskeletal disorders worldwide, yet the available therapies do not provide complete tendon repair/regeneration and full functionality restoring. Extracellular vesicles (EVs), membrane-enclosed nanopresearch articles, have emerged as the next breakthrough in tissue engineering and regenerative medicine to promote endogenous tissue regeneration. Here, we developed a 3D human in vitro model mimicking the signature of pathological tendon and used it to evaluate the influence that different platelet-derived EVs might have in tendon tissue repair mechanisms. For this, different EV populations isolated from platelets, small EVs (sEVs) and medium EVs (mEVs), were added to the culture media of human tendon-derived cells (hTDCs) cultured on isotropic nanofibrous scaffolds. The platelet-derived EVs increased the expression of tenogenic markers, promoted a healthy extracellular matrix (ECM) remodeling, and the synthesis of anti-inflammatory mediators. These findings suggest that platelet EVs provided relevant biochemical cues that potentiated a recovery of hTDCs phenotype from a diseased to a healthy state. Thus, this study opens new perspectives for the translation of platelet-derived EVs as therapeutics.
eng
Extracellular vesicles
Platelets
Tendinopathy
Tendon-derived cells
In vitro models
Fibers
Tissue engineering
Therapeutic Effects of Platelet-Derived Extracellular Vesicles in a Bioengineered Tendon Disease Model
research article