2026-04-29T04:09:49Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/235162024-11-29T06:56:19Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967col_20.500.12105_16983

00925njm 22002777a 4500 dc Bento, Leyre author Vögler, Oliver author Sas-Barbeito, Adriana author Muncunill Farreny, Josep author Ros, Teresa author Martinez, Jordi author Quintero Duarte, Adriana Marcela author Ramos-Asensio, Rafael author Asensio, Víctor José author Fernández-Rodríguez, Concepción author Salar, Antonio author Navarro, Alfons author del Campo Garcia, Raquel author Ibarra de la Rosa, Javier M author Alemany, Regina author Gutierrez, Antonio author 2022-02-20 Diffuse large B cell lymphoma (DLBCL) treatment with R-CHOP regimen produces 5-year progression-free survival and overall survival of around 60-70%. Our objective was to discover prognostic biomarkers allowing early detection of the remaining 30-40% with poor long-term outcome. For this purpose, we applied a novel strategy: from a cohort of DLBCL patients, treated with standard therapy, a discovery group of 12 patients with poor prognosis (advanced stage III-IV, R-IPI > 2) was formed, consisting of six chemoresistant (refractory/early relapse < 12 months) and six chemosensitive (complete remission > 3 years) subjects. By using microarray assays, the most differentially expressed miRNAs were defined as an initial set of prognostic miRNA candidates. Their expression was then analyzed in a validation cohort of 68 patients and the three miRNAs with the most significant impact on event-free and overall survival were selected. In the DLBCL cell line U-2932 the transfection with miR-1244 and miR-193b-5p, but not miR-1231, blocked the effect of CHOP on cell viability. A subsequent gene set enrichment analysis in patients revealed the implication of the first two miRNAs in cell cycle control and chemoresistance-related pathways, whereas the last one was involved in immunological processes. In conclusion, this novel strategy identified three promising prognostic markers for DLBCL patients at high risk of failure with standard therapy. Bento L, Vögler O, Sas-Barbeito A, Muncunill J, Ros T, Martínez J, et al. Screening for Prognostic microRNAs Associated with Treatment Failure in Diffuse Large B Cell Lymphoma. Cancers (Basel). 2022 Feb 20;14(4). 2072-6694 http://hdl.handle.net/20.500.13003/18271 https://hdl.handle.net/20.500.12105/23516 35205813 10.3390/cancers14041065 Cancers 2-s2.0-85124950570 764830100001 L2015725619 Screening for Prognostic microRNAs Associated with Treatment Failure in Diffuse Large B Cell Lymphoma