2026-04-28T22:57:10Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/234992024-11-28T22:40:50Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967

00925njm 22002777a 4500 dc Maimo-Barcelo, Albert author Martin-Saiz, Lucia author Fernández, José Andrés author Perez-Romero, Karim author Garfias-Arjona, Santiago author Lara-Almunia, Monica author Pierola-Lopetegui, Javier author Bestard-Escalas, Joan author Barcelo-Coblijn, Gwendolyn author 2022-03 Glioblastoma (GBM) represents one of the deadliest tumors owing to a lack of effective treatments. The adverse outcomes are worsened by high rates of treatment discontinuation, caused by the severe side effects of temozolomide (TMZ), the reference treatment. Therefore, understanding TMZ's effects on GBM and healthy brain tissue could reveal new approaches to address chemotherapy side effects. In this context, we have previously demonstrated the membrane lipidome is highly cell type-specific and very sensitive to pathophysiological states. However, little remains known as to how membrane lipids participate in GBM onset and progression. Hence, we employed an ex vivo model to assess the impact of TMZ treatment on healthy and GBM lipidome, which was established through imaging mass spectrometry techniques. This approach revealed that bioactive lipid metabolic hubs (phosphatidylinositol and phosphatidylethanolamine plasmalogen species) were altered in healthy brain tissue treated with TMZ. To better understand these changes, we interrogated RNA expression and DNA methylation datasets of the Cancer Genome Atlas database. The results enabled GBM subtypes and patient survival to be linked with the expression of enzymes accounting for the observed lipidome, thus proving that exploring the lipid changes could reveal promising therapeutic approaches for GBM, and ways to ameliorate TMZ side effects. Maimo-Barcelo A, Martin-Saiz L, Fernandez JA, Perez-Romero K, Garfias-Arjona S, Lara-Almunia M, et al. Polyunsaturated Fatty Acid-Enriched Lipid Fingerprint of Glioblastoma Proliferative Regions Is Differentially Regulated According to Glioblastoma Molecular Subtype. Int J Mol Sci. 2022 Mar;23(6):2949. https://hdl.handle.net/20.500.13003/19754 https://hdl.handle.net/20.500.12105/23499 35328369 10.3390/ijms23062949 1422-0067 International Journal of Molecular Sciences 775262100001 Glioblastoma MALDI-IMS lipidomics Temozolomide Modular gene expression Molecular subtypes Lipid metabolism Polyunsaturated Fatty Acid-Enriched Lipid Fingerprint of Glioblastoma Proliferative Regions Is Differentially Regulated According to Glioblastoma Molecular Subtype