2026-04-29T20:21:18Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/234312024-11-28T21:12:01Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967
Repisalud
author
Antich-Rosselló, Miquel
author
Munar-Bestard, Marta
author
Forteza-Genestra, Maria Antónia
author
Calvo Benito, Javier
author
Gayà, Antoni
author
Monjo, Marta
author
Ramis, Joana Maria
2024-10-04T13:22:48Z
2024-10-04T13:22:48Z
2022-07-11
Antich-Rosselló M, Munar-Bestard M, Forteza-Genestra MA, Calvo J, Gayà A, Monjo M, et al. Evaluation of Platelet-Derived Extracellular Vesicles in Gingival Fibroblasts and Keratinocytes for Periodontal Applications. Int J Mol Sci. 2022 Jul 11;23(14):7668.
10.3390/ijms23147668
1422-0067
International journal of molecular sciences
http://hdl.handle.net/20.500.13003/18126
35887008
L2018307945
2-s2.0-85135103611
https://hdl.handle.net/20.500.12105/23431
Gingival regeneration aims at restoring the architecture and functionality of oral damaged tissue. Different biomaterials or biological materials have been tested for tissue repair, such as platelet concentrates such as PL. In this article, the use of extracellular vesicles (EVs) derived from platelet lysate (PL) and their combination with hyaluronic acid biomaterials (HA) in an in vitro wound healing assay is investigated. EVs were isolated by size exclusion chromatography from PL. In addition, HA gels were formulated with PL or EVs. EVs or HA combined with EVs (HA-EVs) were tested in vitro in gingival fibroblasts and keratinocytes for biocompatibility (LDH activity and metabolic activity) and by an in vitro wound-healing assay and gene expression analysis. EVs and EVs-HA treatments were biocompatible in gingival fibroblasts and keratinocytes and showed an increase in wound healing in vitro compared to control. Moreover, changes in gene expression related to extracellular matrix remodeling were observed after the treatment with EVs. EVs can be combined with HA biomaterials, showing good biocompatibility and preserving their activity and functionality. Therefore, platelet-derived EVs could emerge as a new application for periodontal regeneration in combination with biomaterials in order to enhance their clinical use.
eng
Evaluation of Platelet-Derived Extracellular Vesicles in Gingival Fibroblasts and Keratinocytes for Periodontal Applications
research article