2026-05-20T04:33:49Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/234102024-11-28T22:13:13Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967col_20.500.12105_16971
Repisalud
author
Rivaya, Belen
author
Le Roy, Chloe
author
Jordana-Lluch, Elena
author
Fernandez-Rivas, Gema
author
Casan, Cristina
author
Gonzalez, Victoria
author
Wang-Wang, Jun Hao
author
Bebear, Cecile
author
Matas, Lurdes
author
Pereyre, Sabine
2024-10-04T13:16:36Z
2024-10-04T13:16:36Z
2022-04
Rivaya B, Le Roy C, Jordana-Lluch E, Fernandez-Rivas G, Casan C, Gonzalez V, et al. Detection and Prevalence of Macrolide and Fluoroquinolone Resistance in Mycoplasma genitalium in Badalona, Spain. Antibiotics-Basel. 2022 Apr;11(4):485.
10.3390/antibiotics11040485
2079-6382
Antibiotics-Basel
https://hdl.handle.net/20.500.13003/19530
35453236
L2016414761
2-s2.0-85128472828
https://hdl.handle.net/20.500.12105/23410
785540700001
Macrolide and fluoroquinolone resistance (MLr/FQr) in Mycoplasma genitalium (MG) infections is concerning worldwide. Current guidelines recommend performing MLr detection in MG-positive cases to adjust antimicrobial therapy. We aimed to evaluate the usefulness of PCR followed by pyrosequencing for MLr detection in comparison with a one-step commercial assay and to assess the prevalence of MLr and FQr in Badalona, Spain. A total of 415 MG-positive samples by Allplex STI-7 (Seegene) were analyzed for MLr detection by pyrosequencing. From those, 179 samples were further analyzed for MG and MLr by ResistancePlus® MG kit (SpeeDx) and 100 of them also for fluoroquinolone resistance (FQr) by sequencing the parC gene. Regarding MG detection, Allplex and Resistance Plus® showed an overall agreement of 87%, but this value rose to 95.4% if we compare them for MLr detection. Prevalence of MLr was 23.1% in Badalona, but this rate increased to 73.7% in the HIV-positive patients cohort. FQr detection showed 3% of resistant strains. Pyrosequencing is a convenient and cheap technique for MLr detection, but one-step tools should be considered in high-throughput laboratories. Despite the fact that MLr remained moderate and FQr was low in our study, simultaneous MG and MLr detection would improve patient's management applying resistance-guided treatment strategies.
eng
Mycoplasma genitalium
Macrolide
Fluoroquinolone
Resistance
Spain
Detection and Prevalence of Macrolide and Fluoroquinolone Resistance in Mycoplasma genitalium in Badalona, Spain
research article