2026-08-05T16:12:48Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/234072024-11-28T23:11:35Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967
Repisalud
author
Antich-Rosselló, Miquel
author
Forteza-Genestra, Maria Antonia
author
Calvo Benito, Javier
author
Gaya, Antoni
author
Monjo, Marta
author
Ramis, Joana Maria
2024-10-04T13:16:34Z
2024-10-04T13:16:34Z
2022-05-05
Antich-Rossello M, Forteza-Genestra MA, Calvo J, Gaya A, Monjo M, Ramis JM. Customizing the extracellular vesicles release and effect by strategizing surface functionalization of titanium. Sci Rep. 2022 May 5;12(1):7399.
10.1038/s41598-022-11475-3
2045-2322
Scientific Reports
https://hdl.handle.net/20.500.13003/19519
35513419
L637939832
2-s2.0-85129345763
https://hdl.handle.net/20.500.12105/23407
791506100063
Metallic material functionalization with Extracellular Vesicles (EVs) is a desirable therapeutic approach to improve regenerative procedures. Among the different functionalization strategies available, here we have compared drop casting on machined Ti surfaces, drop casting on nanostructured TiO2 surfaces and polymeric entrapment with polydopamine. EVs are a heterogeneous population of communication nanovesicles released by cells that are being intensively investigated for their use in therapeutics. We have selected platelet derived EVs for Ti surface coating due to their demonstrated osteoinductive properties. Our results show that each functionalization strategy leads to differences in the size of EV populations attached to and released from the metallic implants, which, in turn, leads to variations in their osteogenic capability measured through alkaline phosphatase activity and calcium deposition. In conclusion, the functionalization strategy used has an important effect on the resulting implant functionality, probably due to the heterogeneous EVs nature. Thus, the methodological approach to metallic material functionalization should be carefully chosen when working with extracellular vesicles in order to obtain the desired therapeutic application.
eng
Customizing the extracellular vesicles release and effect by strategizing surface functionalization of titanium
research article