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oai:repisalud.isciii.es:20.500.12105/234072024-11-28T23:11:35Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967

00925njm 22002777a 4500 dc Antich-Rosselló, Miquel author Forteza-Genestra, Maria Antonia author Calvo Benito, Javier author Gaya, Antoni author Monjo, Marta author Ramis, Joana Maria author 2022-05-05 Metallic material functionalization with Extracellular Vesicles (EVs) is a desirable therapeutic approach to improve regenerative procedures. Among the different functionalization strategies available, here we have compared drop casting on machined Ti surfaces, drop casting on nanostructured TiO2 surfaces and polymeric entrapment with polydopamine. EVs are a heterogeneous population of communication nanovesicles released by cells that are being intensively investigated for their use in therapeutics. We have selected platelet derived EVs for Ti surface coating due to their demonstrated osteoinductive properties. Our results show that each functionalization strategy leads to differences in the size of EV populations attached to and released from the metallic implants, which, in turn, leads to variations in their osteogenic capability measured through alkaline phosphatase activity and calcium deposition. In conclusion, the functionalization strategy used has an important effect on the resulting implant functionality, probably due to the heterogeneous EVs nature. Thus, the methodological approach to metallic material functionalization should be carefully chosen when working with extracellular vesicles in order to obtain the desired therapeutic application. Antich-Rossello M, Forteza-Genestra MA, Calvo J, Gaya A, Monjo M, Ramis JM. Customizing the extracellular vesicles release and effect by strategizing surface functionalization of titanium. Sci Rep. 2022 May 5;12(1):7399. 10.1038/s41598-022-11475-3 2045-2322 Scientific Reports https://hdl.handle.net/20.500.13003/19519 35513419 L637939832 2-s2.0-85129345763 https://hdl.handle.net/20.500.12105/23407 791506100063 Customizing the extracellular vesicles release and effect by strategizing surface functionalization of titanium