2026-04-29T07:21:30Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/233912024-11-29T07:18:18Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967
Repisalud
author
Rodas, Lluis
author
Martinez, Sonia
author
Riera-Sampol, Aina
author
Moir, Hannah J
author
Tauler, Pedro
author
Rodas, Lluis
author
Martinez, Sonia
author
Riera-Sampol, Aina
author
Moir, Hannah J
author
Tauler, Pedro
2024-10-04T13:16:26Z
2024-10-04T13:16:26Z
2022-01
Rodas L, Martinez S, Riera-Sampol A, Moir HJ, Tauler P. Blood Cell In Vitro Cytokine Production in Response to Lipopolysaccharide Stimulation in a Healthy Population: Effects of Age, Sex, and Smoking. Cells. 2022 Jan;11(1):103.
https://hdl.handle.net/20.500.13003/19470
https://hdl.handle.net/20.500.12105/23391
35011664
10.3390/cells11010103
2073-4409
Cells
2-s2.0-85121824218
758699700001
L2015118454
Immune system functionality has been commonly assessed by a whole-blood or isolated-cell stimulation assay. The aim of this study was to determine whether cytokine production in whole-blood-stimulated samples is influenced by age, sex, and smoking. A descriptive cross-sectional study in 253 healthy participants aged 18-55 years was conducted. Whole blood samples were stimulated for 24 h with LPS and concentrations of IL-6, IL-10, and TNF-α were determined in the culture media. Among parameters considered, statistical regression analysis indicated that smoking (change in R2 = 0.064, p < 0.001) and sex (change in R2 = 0.070, p < 0.001) were the main predictors for IL-10 production, with higher values for women and non-smokers. Age was also found to be a significant predictor (change in R2 = 0.021, p < 0.001), with higher values for younger ages. Age (change in R2 = 0.089, p = 0.013) and smoking (change in R2 = 0.037, p = 0.002) were found to be negative predictors for IL-6 production. Regarding TNF-α-stimulated production, age (change in R2 = 0.029, p = 0.009) and smoking (change in R2 = 0.022, p = 0.022) were found to be negative predictors. Furthermore, sex (change in R2 = 0.016, p = 0.045) was found to be a significant predictor, with lower values for women. In conclusion, sex, age, and smoking were found to be independent determinants of stimulated cytokine production. While female sex is associated with higher IL-10 and lower TNF-α production, aging and smoking are associated with lower IL-6, IL-10, and TNF-α production.
eng
LPS challenge
Blood culture
Age
Sex
Smoking
Menstrual cycle
Blood Cell In Vitro Cytokine Production in Response to Lipopolysaccharide Stimulation in a Healthy Population: Effects of Age, Sex, and Smoking
research article