2026-07-23T17:12:26Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/233562024-11-29T04:03:04Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967
Repisalud
author
Yau-Qiu, Zhi Xin
author
Madrid-Gambin, Francisco
author
Brennan, Lorraine
author
Palou, Andreu
author
Rodriguez, Ana Maria
2024-09-18T06:45:06Z
2024-09-18T06:45:06Z
2021-08
Yau-Qiu ZX, Madrid-Gambin F, Brennan L, Palou A, Rodriguez AM. Leptin Supplementation During Lactation Restores Key Liver Metabolite Levels Malprogrammed by Gestational Calorie Restriction. Mol Nutr Food Res. 2021 Aug;65(16):2001046.
10.1002/mnfr.202001046
1613-4133
1613-4125
Molecular Nutrition & Food Research
https://hdl.handle.net/20.500.13003/19666
33900028
L634883258
2-s2.0-85111796250
https://hdl.handle.net/20.500.12105/23356
680066000001
Introduction: Perinatal nutritional factors can program offspring metabolic phenotype and risk to obesity. This study investigates the potential role of leptin supplementation (during lactation) in ameliorating the malprogrammed effects caused by mild maternal calorie restriction during gestation, on young rat offspring liver metabolic response. Methods and Results: Untargeted and targeted metabolomics studies on liver samples are performed by NMR and GC-MS, respectively. Global DNA methylation and the expression by RT-PCR of key genes involved in different pathways are also determined. By NMR, 15 liver metabolites are observed to be altered in the offspring of gestational calorie-restricted dams (CR group), at days 25-27 of life. Physiological leptin supplementation during lactation partially reverted the effect of CR condition for most of these metabolites. Moreover, targeted fatty acid analysis by GC-MS shows a significant decrease in the hepatic concentration of certain very long-chain fatty acids (VLCFA) in CR offspring, partially or totally reverted by leptin supplementation. No remarkable changes are found in global DNA methylation or mRNA expression. Conclusion: Physiological leptin supplementation during lactation contributes to the reversion of changes caused by maternal mild calorie restriction on the liver metabolome. This agrees with a putative role of leptin supplementation preventing or reversing metabolic disturbances caused by gestational metabolic malprogramming.
eng
Calorie restriction
Leptin
Liver
Metabolic programming
Perinatal nutrition
Leptin Supplementation During Lactation Restores Key Liver Metabolite Levels Malprogrammed by Gestational Calorie Restriction
research article