2026-07-23T17:12:25Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/233562024-11-29T04:03:04Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967

00925njm 22002777a 4500 dc Yau-Qiu, Zhi Xin author Madrid-Gambin, Francisco author Brennan, Lorraine author Palou, Andreu author Rodriguez, Ana Maria author 2021-08 Introduction: Perinatal nutritional factors can program offspring metabolic phenotype and risk to obesity. This study investigates the potential role of leptin supplementation (during lactation) in ameliorating the malprogrammed effects caused by mild maternal calorie restriction during gestation, on young rat offspring liver metabolic response. Methods and Results: Untargeted and targeted metabolomics studies on liver samples are performed by NMR and GC-MS, respectively. Global DNA methylation and the expression by RT-PCR of key genes involved in different pathways are also determined. By NMR, 15 liver metabolites are observed to be altered in the offspring of gestational calorie-restricted dams (CR group), at days 25-27 of life. Physiological leptin supplementation during lactation partially reverted the effect of CR condition for most of these metabolites. Moreover, targeted fatty acid analysis by GC-MS shows a significant decrease in the hepatic concentration of certain very long-chain fatty acids (VLCFA) in CR offspring, partially or totally reverted by leptin supplementation. No remarkable changes are found in global DNA methylation or mRNA expression. Conclusion: Physiological leptin supplementation during lactation contributes to the reversion of changes caused by maternal mild calorie restriction on the liver metabolome. This agrees with a putative role of leptin supplementation preventing or reversing metabolic disturbances caused by gestational metabolic malprogramming. Yau-Qiu ZX, Madrid-Gambin F, Brennan L, Palou A, Rodriguez AM. Leptin Supplementation During Lactation Restores Key Liver Metabolite Levels Malprogrammed by Gestational Calorie Restriction. Mol Nutr Food Res. 2021 Aug;65(16):2001046. 10.1002/mnfr.202001046 1613-4133 1613-4125 Molecular Nutrition & Food Research https://hdl.handle.net/20.500.13003/19666 33900028 L634883258 2-s2.0-85111796250 https://hdl.handle.net/20.500.12105/23356 680066000001 Calorie restriction Leptin Liver Metabolic programming Perinatal nutrition Leptin Supplementation During Lactation Restores Key Liver Metabolite Levels Malprogrammed by Gestational Calorie Restriction