2026-04-29T04:09:09Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/233532024-11-28T23:37:57Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967
Repisalud
author
Pomar, Catalina A
author
Serra, Francisca
author
Palou, Andreu
author
Sanchez, Juana
2024-09-18T06:45:05Z
2024-09-18T06:45:05Z
2021-10
Pomar CA, Serra F, Palou A, Sanchez J. Lower miR-26a levels in breastmilk affect gene expression in adipose tissue of offspring. Faseb J. 2021 Oct;35(10):e21924.
0892-6638
https://hdl.handle.net/20.500.13003/19674
https://hdl.handle.net/20.500.12105/23353
34582059
10.1096/fj.202100623R
1530-6860
Faseb Journal
2-s2.0-85115851797
702238200019
L2013818453
Breastmilk miRNAs may act as epigenetic regulators of metabolism and energy homeostasis in offspring. Here, we aimed to investigate the regulatory effects of miR-26a on adipose tissue development. First, the 3T3-L1 cell model was used to identify putative target genes for miR-26a. Then, target genes were analysed in adipose tissue of offspring from dams that supplied lower levels of breastmilk miR-26a to determine whether miR-26a milk concentration might have a long-lasting impact on adipose tissue in the progeny. In the in vitro model, both over- and under-expression of miR-26a were induced by transfecting into 3T3-L1 with miR-26a mimic and inhibitor. Array analysis was performed after induction of miR-26a to ascertain the impact on mRNA target genes and influence of differentiation status. Focusing on genes related to adipose tissue development, transfection with miR-26a mimic reduced the expression of Pten, Hmga1, Stk11, Rb1, and Adam17 in both pre- and mature adipocytes. Data mostly confirmed the results found in the animal model. After weaning, descendants of cafeteria-fed dams breastfed with lower levels of miR-26a displayed greater expression of Hmag1, Rb1, and Adam17 in retroperitoneal white adipose tissue in comparison with controls. Hence, alterations in the amount of miR-26a supplied through milk during lactation is able to alter the expression of target genes in the descendants and may affect adipose tissue development. Thus, milk miR-26a may act as an epigenetic regulator influencing early metabolic program in the progeny, which emerges as a relevant component of an optimal milk composition for correct development.
eng
Breastfeeding
Breastmilk
Milk's epigenetic regulators
miR-26a
Lower miR-26a levels in breastmilk affect gene expression in adipose tissue of offspring
research article