2026-05-22T00:44:55Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/233472025-04-08T11:21:25Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967
Repisalud
author
Bis-Humbert, Cristian
author
García-Fuster, M Julia
2024-09-18T06:45:04Z
2024-09-18T06:45:04Z
2021-12
Bis-Humbert C, Garcia-Fuster MJ. Adolescent cocaine induced persistent negative affect in female rats exposed to early-life stress. Psychopharmacology. 2021 Dec;238(12):3399-410.
10.1007/s00213-021-05955-z
1432-2072
0033-3158
Psychopharmacology
https://hdl.handle.net/20.500.13003/19425
34430991
L2013519241
2-s2.0-85113352521
https://hdl.handle.net/20.500.12105/23347
687907400001
Rationale: The combination of several risk factors (sex, a prior underlying psychiatric condition, or early drug initiation) could induce the emergence of negative affect during cocaine abstinence and increase the risk of developing addiction. However, most prior preclinical studies have been centered in male rodents, traditionally excluding females from these analyses. Objectives: To ascertain the behavioral and neurochemical consequences of adolescent cocaine exposure when the combination of several risk factors is present (female, early-life stress). Methods: Whole litters of Sprague-Dawley rats were exposed to maternal deprivation for 24 h on postnatal day (PND) 9. Cocaine was administered in adolescence (15 mg/kg/day, i.p., PND 33-39). Negative affect was assessed by several behavioral tests (forced swim, open field, novelty-suppressed feeding, sucrose preference). Hippocampal cell fate markers were evaluated by western blot (FADD, Bax, cytochrome c) or immunohistochemistry (Ki-67; cell proliferation). Results Maternal deprivation is a suitable model of psychiatric vulnerability in which to study the impact of adolescent cocaine in female rats. While adolescent cocaine did not alter affective-like behavior during adolescence, a pro-depressive-like state emerged during adulthood, exclusively in rats re-exposed to cocaine during abstinence. FADD regulation by cocaine in early-life stressed female rats might contribute to certain hippocampal neuroadaptations with some significance to the observed induced negative affect. Conclusions: Adolescent cocaine induced persistent negative affect in female rats exposed to early-life stress, highlighting the risk of early drug initiation during adolescence for the emergence of negative reinforcement during abstinence likely driving cocaine addiction vulnerability, also in female rats.
eng
Adolescence
Cocaine
Maternal deprivation
Negative affect
Rat
Adolescent cocaine induced persistent negative affect in female rats exposed to early-life stress
research article