2026-08-13T07:18:05Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/232182024-09-22T04:01:20Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967
Repisalud
author
Reynes, Barbara
author
Serra, Francisca
author
Palou, Andreu
2024-09-18T06:42:39Z
2024-09-18T06:42:39Z
2021-04
Reynes B, Serra F, Palou A. Rapid visual detection of SARS-CoV-2 by colorimetric loop-mediated isothermal amplification. Biotechniques. 2021 Apr;70(4):218-25.
10.2144/btn-2020-0159
1940-9818
0736-6205
Biotechniques
https://hdl.handle.net/20.500.13003/19789
33820475
L2011906853
2-s2.0-85104369629
https://hdl.handle.net/20.500.12105/23218
637782500001
Evaluation of the performance of a new set of primers defined from the ORF1ab sequence, and its combination with a previously published set of primers from the N sequence, to detect SARS-CoV-2 RNA by the loop-mediated isothermal amplification technique is presented. The ORF1ab primer set enables visual detection of SARS-CoV-2 RNA in 16 min. In addition, a simultaneous reaction with both ORF1ab and N primers allows for higher sensitivity of detection, particularly when low numbers of copies are present (250 viral RNA copies). Further, the protocol is able to detect viral RNA in saliva samples. The procedure reported could be easily implemented in the generation of a new and sensitive rapid point-of care device for SARS-CoV-2 RNA visual detection. METHOD SUMMARY Optimized loop-mediated isothermal amplification reactions were carried out using the combination of two sets of primers (designed on ORF1ab and N sequences). Saliva samples were pretreated by heating and proteinase K, before SARS-CoV-2 determination by loop-mediated isothermal amplification.
eng
COVID-19
Nucleocapsid primers
ORF1ab primers
SARS-CoV-2 detection
Virus LAMP amplification
Rapid visual detection of SARS-CoV-2 by colorimetric loop-mediated isothermal amplification
research article