2026-05-21T23:28:16Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/230862024-11-29T17:01:10Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597
Repisalud
author
Zimmermann, Fabian
author
Serna, Marina
author
Ezquerra, Artur
author
Fernandez-Leiro, Rafael
author
Llorca Blanco, Oscar Antonio
author
Luders, Jens
author
Zimmermann, Fabian
author
Ezquerra, Artur
author
Fernandez-Leiro, Rafael
author
Luders, Jens
funder
Ministerio de Ciencia, Innovación y Universidades (España)
funder
Instituto de Salud Carlos III
funder
Marie Curie
funder
Agencia de Gestio D'Ajuts Universitaris de Recerca Agaur (AGAUR)
funder
IRB Barcelona
funder
European Union (EU)
funder
La Caixa Foundation
funder
PRB3
2024-09-16T08:16:58Z
2024-09-16T08:16:58Z
2020-12-18
Sci Adv . 2020 ;6(51):eabe0894.
10.1126/sciadv.abe0894
2375-2548
Science advances
33355144
https://hdl.handle.net/20.500.12105/23086
The microtubule nucleator ?-tubulin ring complex (?TuRC) is essential for the function of microtubule organizing centers such as the centrosome. Since its discovery over two decades ago, ?TuRC has evaded in vitro reconstitution and thus detailed structure-function studies. Here, we show that a complex of RuvB-like protein 1 (RUVBL1) and RUVBL2 "RUVBL" controls assembly and composition of ?TuRC in human cells. Likewise, RUVBL assembles ?TuRC from a minimal set of core subunits in a heterologous coexpression system. RUVBL interacts with ?TuRC subcomplexes but is not part of fully assembled ?TuRC. Purified, reconstituted ?TuRC has nucleation activity and resembles native ?TuRC as revealed by its cryo-electron microscopy (cryo-EM) structure at ~4.0-� resolution. We further use cryo-EM to identify features that determine the intricate, higher-order ?TuRC architecture. Our work finds RUVBL as an assembly factor that regulates ?TuRC in cells and allows production of recombinant ?TuRC for future in-depth mechanistic studies.
eng
Assembly of the asymmetric human ?-tubulin ring complex by RUVBL1-RUVBL2 AAA ATPase.
research article