2026-08-13T07:18:20Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/230752024-11-29T14:16:26Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597
Repisalud
author
Guerra, C
author
Roncero, C
author
Porras, A
author
Fernández, M
author
Benito, M
2024-09-16T08:16:55Z
2024-09-16T08:16:55Z
1996-01-26
J Biol Chem . 1996 ;271(4):2076-81.
10.1074/jbc.271.4.2076
0021-9258
The Journal of biological chemistry
8567662
https://hdl.handle.net/20.500.12105/23075
Confluent fetal rat brown adipocytes in primary culture showed an almost undetectable level of uncoupling protein (UCP) mRNA and a low mitochondrial content of functional UCP. Treatment of confluent cells with 10 nM triiodothyronine in a serum-free medium, in the absence of noradrenergic stimulation, increased the amount of UCP mRNA in a time-dependent manner. This effect was due to an increased UCP gene transcription rate and UCP mRNA stabilization, resulting in a higher content of immunoreactive mitochondrial UCP and functional UCP (detected by its ability to bind GDP). Thus, triiodothyronine might play a significant physiological role in the UCP expression throughout fetal development, when brown adipose tissue starts to differentiate and UCP is primarily expressed.
eng
Triiodothyronine induces the transcription of the uncoupling protein gene and stabilizes its mRNA in fetal rat brown adipocyte primary cultures.
research article