2026-04-30T06:48:09Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/230452024-11-29T05:34:56Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967

00925njm 22002777a 4500 dc Kosmac, Kate author Gonzalez-Freire, Marta author McDermott, Mary M author White, Sarah H author Walton, R. Grace author Sufit, Robert L author Tian, Lu author Li, Lingyu author Kibbe, Melina R author Criqui, Michael H author Guralnik, Jack M author Polonsky, Tamar S author Leeuwenburgh, Christiaan author Ferrucci, Luigi author Peterson, Charlotte A author 2020-05-18 BACKGROUND: Peripheral artery disease (PAD) is a manifestation of atherosclerosis characterized by reduced blood flow to the lower extremities and mobility loss. Preliminary evidence suggests PAD damages skeletal muscle, resulting in muscle impairments that contribute to functional decline. We sought to determine whether PAD is associated with an altered macrophage profile in gastrocnemius muscles and whether muscle macrophage populations are associated with impaired muscle phenotype and walking performance in patients with PAD. METHODS AND RESULTS: Macrophages, satellite cells, and extracellular matrix in gastrocnemius muscles from 25 patients with PAD and 7 patients without PAD were quantified using immunohistochemistry. Among patients with PAD, both the absolute number and percentage of cluster of differentiation (CD) 11b+CD206+ M2-like macrophages positively correlated to satellite cell number (r=0.461 [P=0.023] and r=0.416 [P=0.042], respectively) but not capillary density or extracellular matrix. The number of CD11b+CD206- macrophages negatively correlated to 4-meter walk tests at normal (r=-0.447, P=0.036) and fast pace (r=-0.510, P=0.014). Extracellular matrix occupied more muscle area in PAD compared with non-PAD (8.72 +/- 2.19% versus 5.30 +/- 1.03%, P<0.001) and positively correlated with capillary density (r=0.656, P<0.001). CONCLUSIONS: Among people with PAD, higher CD206+ M2-like macrophage abundance was associated with greater satellite cell numbers and muscle fiber size. Lower CD206- macrophage abundance was associated with better walking performance. Further study is needed to determine whether CD206+ macrophages are associated with ongoing reparative processes enabling skeletal muscle adaptation to damage with PAD. Kosmac K, Gonzalez-Freire M, Mcdermott MM, White SH, Walton RG, Sufit RL, et al. Correlations of Calf Muscle Macrophage Content With Muscle Properties and Walking Performance in Peripheral Artery Disease. J Am Heart Assoc. 2020 May 18;9(10):e015929. Epub 2020 May 9. 10.1161/JAHA.118.015929 2047-9980 Journal of the American Heart Association http://hdl.handle.net/20.500.13003/17128 32390569 L631719471 2-s2.0-85084943568 https://hdl.handle.net/20.500.12105/23045 550987100013 Macrophage Peripheral artery disease Skeletal muscle Walking performance Correlations of Calf Muscle Macrophage Content With Muscle Properties and Walking Performance in Peripheral Artery Disease