2026-05-20T03:47:19Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/229602024-11-28T22:36:32Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967col_20.500.12105_16970
Repisalud
author
Bestard-Escalas, Joan
author
Maimó-Barceló, Albert
author
Lopez, Daniel H
author
Reigada, Rebeca
author
Guardiola-Serrano, Francisca
author
Ramos-Vivas, Jose
author
Hornemann, Thorsten
author
Okazaki, Toshiro
author
Barcelo-Coblijn, Gwendolyn
2024-09-13T09:14:41Z
2024-09-13T09:14:41Z
2020-05
Bestard J, Maimó-Barceló A, Lopez DH, Reigada Mendez R, Guardiola-Serrano F, Ramos-Vivas J, et al. Common and Differential Traits of the Membrane Lipidome of Colon Cancer Cell Lines and Their Secreted Vesicles: Impact on Studies Using Cell Lines. Cancers. 2020 May;12(5):1293.
10.3390/cancers12051293
2072-6694
Cancers
http://hdl.handle.net/20.500.13003/10798
32443825
L2004436936
2-s2.0-85085361019
https://hdl.handle.net/20.500.12105/22960
539246000234
Colorectal cancer (CRC) is the fourth leading cause of cancer death in the world. Despite the screening programs, its incidence in the population below the 50s is increasing. Therefore, new stratification protocols based on multiparametric approaches are highly needed. In this scenario, the lipidome is emerging as a powerful tool to classify tumors, including CRC, wherein it has proven to be highly sensitive to cell malignization. Hence, the possibility to describe the lipidome at the level of lipid species has renewed the interest to investigate the role of specific lipid species in pathologic mechanisms, being commercial cell lines, a model still heavily used for this purpose. Herein, we characterize the membrane lipidome of five commercial colon cell lines and their extracellular vesicles (EVs). The results demonstrate that both cell and EVs lipidome was able to segregate cells according to their malignancy. Furthermore, all CRC lines shared a specific and strikingly homogenous impact on ether lipid species. Finally, this study also cautions about the need of being aware of the singularities of each cell line at the level of lipid species. Altogether, this study firmly lays the groundwork of using the lipidome as a solid source of tumor biomarkers.
eng
Colorectal cancer
Lipidomics
Lipid biomarkers
Extracellular vesicles
Cell lines
Plasmalogens
Common and Differential Traits of the Membrane Lipidome of Colon Cancer Cell Lines and Their Secreted Vesicles: Impact on Studies Using Cell Lines
research article