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oai:repisalud.isciii.es:20.500.12105/229532024-11-29T03:49:33Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967
Repisalud author Pons, Daniel-Gabriel author Moran, Carmen author Alorda-Clara, Marina author Oliver, Jordi author Roca, Pilar author Sastre-Serra, Jorge 2024-09-13T09:14:39Z 2024-09-13T09:14:39Z 2020-03-24 Pons DG, Moran C, Alorda-Clara M, Oliver J, Roca P, Sastre-Serra J. Micronutrients Selenomethionine and Selenocysteine Modulate the Redox Status of MCF-7 Breast Cancer Cells. Nutrients. 2020 Mar 24;12(3). http://hdl.handle.net/20.500.13003/18256 https://hdl.handle.net/20.500.12105/22953 32213883 10.3390/nu12030865 2072-6643 Nutrients 2-s2.0-85082487345 531831000274 L2004100643 Selenium is a micronutrient which is found in many foods, with redox status modulation activity. Our aim was to evaluate the effects of two chemical forms of selenoamino acids, Seleno-L-methionine and Seleno-L-cystine (a diselenide derived from selenocysteine), at different concentrations on cell viability, hydrogen peroxide production, antioxidant enzymes, UCP2 protein expression, as well as lipid and protein oxidative damage in MCF-7 breast cancer cells. Results showed that Seleno-L-methionine did not cause an increase in hydrogen peroxide production at relatively low concentrations, accompanied by a rise in the antioxidant enzymes catalase and MnSOD, and UCP2 protein expression levels. Furthermore, a decrease in protein and lipid oxidative damage was observed at 10 µM concentration. Otherwise, Seleno-L-cystine increased hydrogen peroxide production from relatively low concentrations (100 nM) to a large increase at high concentrations. Moreover, at 10 µM, Seleno-L-cystine decreased UCP2 and MnSOD protein expression. In conclusion, the chemical form of selenoamino acid and their incorporation to selenoproteins could affect the regulation of the breast cancer cell redox status. Taken together, the results obtained in this study imply that it is important to control the type of selenium-enriched nutrient consumption, taking into consideration their composition and concentration. eng Micronutrients Selenomethionine and Selenocysteine Modulate the Redox Status of MCF-7 Breast Cancer Cells research article