2026-05-22T01:39:15Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/229392024-11-28T20:30:02Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967
Repisalud
author
Sureda Gomila, Antoni
author
Martorell, Miquel
author
Bibiloni Esteva, Maria Del Mar
author
Bouzas, Cristina
author
Gallardo-Alfaro, Laura
author
Mateos, David
author
Capó Fiol, Xavier
author
Tur, Josep A
author
Pons, Antoni
2024-09-13T09:13:38Z
2024-09-13T09:13:38Z
2020-01-04
Sureda A, Martorell M, Bibiloni MDM, Bouzas C, Gallardo-Alfaro L, Mateos D, et al. Effect of Free Fatty Acids on Inflammatory Gene Expression and Hydrogen Peroxide Production by Ex Vivo Blood Mononuclear Cells. Nutrients. 2020 Jan 4;12(1).
10.3390/nu12010146
2072-6643
Nutrients
http://hdl.handle.net/20.500.13003/18237
31947975
L2003459656
2-s2.0-85077572916
https://hdl.handle.net/20.500.12105/22939
516825500146
The aim of this study was to assess free fatty acids' (FAs) ex vivo anti-/proinflammatory capabilities and their influence on inflammatory gene expression and H2O2 production by human peripheral blood mononuclear cells (PBMCs). Anthropometric and clinical measurements were performed in 26 participants with metabolic syndrome. Isolated PBMCs were incubated ex vivo for 2 h with several free fatty acids-palmitic, oleic, α-linolenic, γ-linolenic, arachidonic and docosahexaenoic at 50 μM, and lipopolysaccharide (LPS) alone or in combination. H2O2 production and IL6, NFκB, TLR2, TNFα, and COX-2 gene expressions were determined. Palmitic, γ-linolenic, and arachidonic acids showed minor effects on inflammatory gene expression, whereas oleic, α-linolenic, and docosahexaenoic acids reduced proinflammatory gene expression in LPS-stimulated PBMCs. Arachidonic and α-linolenic acids treatment enhanced LPS-stimulated H2O2 production by PBMCs, while palmitic, oleic, γ-linolenic, and docosahexaenoic acids did not exert significant effects. Oleic, α-linolenic, and docosahexaenoic acids induced anti-inflammatory responses in PBMCs. Arachidonic and α-linolenic acids enhanced the oxidative status of LPS-stimulated PBMCs. In conclusion, PBMC ex vivo assays are useful to assess the anti-/proinflammatory and redox-modulatory effects of fatty acids or other food bioactive compounds.
eng
Effect of Free Fatty Acids on Inflammatory Gene Expression and Hydrogen Peroxide Production by Ex Vivo Blood Mononuclear Cells
research article