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oai:repisalud.isciii.es:20.500.12105/229372024-11-28T22:15:21Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967

00925njm 22002777a 4500 dc Soldevila Verdeguer, Carla author Martorell, Miquel author Bibiloni Esteva, Maria Del Mar author Bouzas, Cristina author Gallardo-Alfaro, Laura author Mateos, David author Capó Fiol, Xavier author Tur, Josep A author Pons, Antoni author 2020-01 The aim of this study was to assess free fatty acids' (FAs) ex vivo anti-/proinflammatory capabilities and their influence on inflammatory gene expression and H2O2 production by human peripheral blood mononuclear cells (PBMCs). Anthropometric and clinical measurements were performed in 26 participants with metabolic syndrome. Isolated PBMCs were incubated ex vivo for 2 h with several free fatty acids-palmitic, oleic, alpha-linolenic, gamma-linolenic, arachidonic and docosahexaenoic at 50 mu M, and lipopolysaccharide (LPS) alone or in combination. H2O2 production and IL6, NF kappa B, TLR2, TNF alpha, and COX-2 gene expressions were determined. Palmitic, gamma-linolenic, and arachidonic acids showed minor effects on inflammatory gene expression, whereas oleic, alpha-linolenic, and docosahexaenoic acids reduced proinflammatory gene expression in LPS-stimulated PBMCs. Arachidonic and alpha-linolenic acids treatment enhanced LPS-stimulated H2O2 production by PBMCs, while palmitic, oleic, gamma-linolenic, and docosahexaenoic acids did not exert significant effects. Oleic, alpha-linolenic, and docosahexaenoic acids induced anti-inflammatory responses in PBMCs. Arachidonic and alpha-linolenic acids enhanced the oxidative status of LPS-stimulated PBMCs. In conclusion, PBMC ex vivo assays are useful to assess the anti-/proinflammatory and redox-modulatory effects of fatty acids or other food bioactive compounds. Sureda A, Martorell M, Bibiloni MM, Bouzas C, Gallardo-Alfaro L, Mateos D, et al. Effect of Free Fatty Acids on Inflammatory Gene Expression and Hydrogen Peroxide Production by Ex Vivo Blood Mononuclear Cells. Nutrients. 2020 Jan;12(1):146. 10.3390/nu12010146 2072-6643 Nutrients http://hdl.handle.net/20.500.13003/12420 31947975 L2003459656 2-s2.0-85077572916 https://hdl.handle.net/20.500.12105/22937 516825500146 PBMC Fatty acids LPS Gene expression ROS production Effect of Free Fatty Acids on Inflammatory Gene Expression and Hydrogen Peroxide Production by Ex Vivo Blood Mononuclear Cells