2026-05-22T02:42:42Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/228452024-11-29T01:58:52Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967

00925njm 22002777a 4500 dc Alba-Patino, Alejandra author Adrover-Jaume, Cristina author de la Rica, Roberto author 2020-01 Biosensors made entirely of paper are becoming increasingly popular due to their low cost, facile fabrication, and lightweight portability for in-field measurements. However, it is difficult to store nanoparticles in paper substrates without irreversibly binding them to the cellulose matrix. This makes it challenging to fabricate biosensors incorporating nanoparticle probes in paper-based reservoirs. Here, we overcome this limitation with a new method for storing protein-decorated nanoparticles on paper substrates that also allows to release them on demand. It consists of spotting nanoparticles onto pieces of filter paper previously modified with polystyrene sulfonate. Gold nanoparticles modified with avidin or antibodies can be easily transferred from the dry reservoir to a receiving wet piece of paper by simply pressing with the finger or a clamp. Paper-based immunosensors incorporating the reservoir enabled the detection of glycoprotein B from human cytomegalovirus in serum with a limit of detection of 0.03 ng mL(-1) and a total assay time of only 12 min. The low limit of detection obtained with a short assay time along with the long shelf-life of the reservoirs make the proposed paper-only biosensors ideal of point-of-care diagnostics. Alba Patiño Alejandra, Adrover Jaume Cristina, De La Rica Roberto. Nanoparticle Reservoirs for Paper-Only Immunosensors. ACS Sens. 2020 Jan;5(1):147-153. Epub 2020 Jan 6. 10.1021/acssensors.9b01937 2379-3694 Acs Sensors http://hdl.handle.net/20.500.13003/12465 31850749 L630308117 2-s2.0-85078151335 https://hdl.handle.net/20.500.12105/22845 510079300021 Biosensor Origami Gold Colorimetric Plasmonic Paper-based analytical devices Sepsis Nanoparticle Reservoirs for Paper-Only Immunosensors