2026-06-14T02:29:08Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/227772024-11-29T01:53:02Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967

00925njm 22002777a 4500 dc Torrens, Gabriel author Bover Barceló, Isabel author Pérez-Gallego, Marcelo author Escobar-Salom, Maria author Tur-Gracia, Sara author Munar-Bestard, Marta author González-Nicolau, María Del Mar author Cabrera-Venegas, Yoandy José author Rigo-Rumbos, Estefany Nayariyh author Cabot, Gabriel author López-Causapè, Carla author Rojo Molinero, Estrella author Oliver, Antonio author Juan, Carlos author 2019-03-05 In the current scenario of high antibiotic resistance, the search for therapeutic options against Pseudomonas aeruginosa must be approached from different perspectives: cell-wall biology as source of bacterial weak points and our immune system as source of weapons. Our recent study suggests that once the permeability barrier has been overcome, the activity of our cell-wall-targeting immune proteins is notably enhanced, more in mutants with impaired peptidoglycan recycling. The present work aims at analyzing the activity of these proteins [lysozyme and Peptidoglycan-Recognition-Proteins (PGLYRPs)], alone or with a permeabilizer (subinhibitory colistin) in clinical strains, along with other features related to the cell-wall. We compared the most relevant and complementary scenarios: acute (bacteremia) and chronic infections [early/late isolates from lungs of cystic fibrosis (CF) patients]. Although a low activity of lysozyme/PGLYRPs per se (except punctual highly susceptible strains) was found, the colistin addition significantly increased their activity regardless of the strains' colistin resistance levels. Our results show increased susceptibility in late CF isolates, suggesting that CF adaptation renders P. aeruginosa more vulnerable to proteins targeting the cell-wall. Thus, our work suggests that attacking some P. aeruginosa cell-wall biology-related elements to increase the activity of our innate weapons could be a promising therapeutic strategy. Torrens Ribot G, Barcelo Munar IM, Perez-Gallego M, Escobar Salom M, Tur-Gracia S, Munar Bestard M, et al. Profiling the susceptibility of Pseudomonas aeruginosa strains from acute and chronic infections to cell-wall-targeting immune proteins. Sci Rep. 2019 Mar 05;9:3575. 10.1038/s41598-019-40440-w 2045-2322 Scientific Reports http://hdl.handle.net/20.500.13003/17596 30837659 L626688002 2-s2.0-85062585213 https://hdl.handle.net/20.500.12105/22777 460381600148 Profiling the susceptibility of Pseudomonas aeruginosa strains from acute and chronic infections to cell-wall-targeting immune proteins