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oai:repisalud.isciii.es:20.500.12105/227552024-11-29T04:05:55Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967

00925njm 22002777a 4500 dc Ramis, Rafael author Ortega-Castro, Joaquin author Caballero, Carmen author Casasnovas, Rodrigo author Cerrillo, Antonia author Vilanova, Bartolome author Adrover, Miquel author Frau, Juan author 2019-09 Pyridoxamine, one of the natural forms of vitamin B-6, is known to be an effective inhibitor of the formation of advanced glycation end products (AGEs), which are closely related to various human diseases. Pyridoxamine forms stable complexes with metal ions that catalyze the oxidative reactions taking place in the advanced stages of the protein glycation cascade. It also reacts with reactive carbonyl compounds generated as byproducts of protein glycation, thereby preventing further protein damage. We applied Density Functional Theory to study the primary antioxidant activity of pyridoxamine towards three oxygen-centered radicals (center dot OOH, center dot OOCH3 and center dot OCH3) to find out whether this activity may also play a crucial role in the context of protein glycation inhibition. Our results show that, at physiological pH, pyridoxamine can trap the center dot OCH3 radical, in both aqueous and lipidic media, with rate constants in the diffusion limit (>1.0 x 10(8) M-1 s-1). The quickest pathways involve the transfer of the hydrogen atoms from the protonated pyridine nitrogen, the protonated amino group or the phenolic group. Its reactivity towards center dot OOH and center dot OOCH3 is smaller, but pyridoxamine can still scavenge them with moderate rate constants in aqueous media. Since reactive oxygen species are also involved in the formation of AGEs, these results highlight that the antioxidant capacity of pyridoxamine is also relevant to explain its inhibitory role on the glycation process. Ramis Cortés R, Ortega-Castro J, Caballero C, Casanovas R, Cerrillo A, Vilanova Canet B, et al. How Does Pyridoxamine Inhibit the Formation of Advanced Glycation End Products? The Role of Its Primary Antioxidant Activity. Antioxidants. 2019 Sep;8(9):344. 10.3390/antiox8090344 2076-3921 Antioxidants http://hdl.handle.net/20.500.13003/15153 31480509 2-s2.0-85073324606 https://hdl.handle.net/20.500.12105/22755 487957300058 Pyridoxamine DFT AGEs Inhibition ROS How Does Pyridoxamine Inhibit the Formation of Advanced Glycation End Products? The Role of Its Primary Antioxidant Activity