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oai:repisalud.isciii.es:20.500.12105/226562024-11-28T16:35:50Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16939col_20.500.12105_16961col_20.500.12105_16967col_20.500.12105_16983col_20.500.12105_16972

00925njm 22002777a 4500 dc Millares, Laura author Pascual, Sergi author Monton, Concepcion author Garcia-Nunez, Marian author Lalmolda, Cristina author Faner, Rosa author Casadevall, Carme author Seto, Laia author Capilla, Silvia author Moreno, Amalia author Angelica Castro-Acosta, Ady author Jose Alvarez-Martinez, Carlos author Sibila, Oriol author Peces-Barba, German author García-Cosío, Borja author Agusti, Alvar author Gea, Joaquim author Monso, Eduard author 2019-06-24 Background: The respiratory microbiome is altered in COPD patients but its relationship with core components of the disease, such as the severity of airflow limitation, the frequency of exacerbations or the circulating levels of eosinophils, is unclear. Methods: Cross-sectional study comprising 72 clinically stable COPD patients (mean age 68 [SD 7.9] years; FEV1 48.7 [SD 20.1]% of reference) who provided spontaneous sputum samples for 16S rRNA gene amplification and sequencing. The microbiome composition was analysed with QIIME. Results: We observed that: (1) more severe airflow limitation was associated with reduced relative abundance (RA) of Treponema and an increase in Pseudomonas; (2) patients with 2 exacerbations the previous year showed a significantly different bacterial community with respect to non-exacerbators (p=0.014), with changes in 13 genera, including an increase of Pseudomonas, and finally, (3) peripheral eosinophils levels 2% were associated with more diverse microbiome [Chao1 224.51 (74.88) vs 277.39 (78.92) p=0.006; Shannon 3.94 (1.05) vs 4.54 (1.06) p=0.020], and a significant increase in the RAs of 20 genera. Conclusion: The respiratory microbiome in clinically stable COPD patients varies significantly according to the severity of airflow limitation, previous history of exacerbations and circulating eosinophils levels. Millares L, Pascual S, Monton C, Garcia-Nunez M, Lalmolda C, Faner R, et al. Relationship between the respiratory microbiome and the severity of airflow limitation, history of exacerbations and circulating eosinophils in COPD patients. BMC Pulm Med. 2019 Jun 24;19:112. 10.1186/s12890-019-0867-x 1471-2466 BMC Pulmonary Medicine http://hdl.handle.net/20.500.13003/16297 31234826 L628244679 2-s2.0-85068169882 https://hdl.handle.net/20.500.12105/22656 472880900001 Bacterial community Diversity Eosinophils Exacerbations Sputum Stable COPD Relationship between the respiratory microbiome and the severity of airflow limitation, history of exacerbations and circulating eosinophils in COPD patients