2026-08-05T15:43:25Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/226222025-04-08T10:08:34Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16958col_20.500.12105_16962col_20.500.12105_16967

00925njm 22002777a 4500 dc Moura, David S author Ramos-Asensio, Rafael author Fernández-Serra, Antonio author Serrano, Teresa author Cruz, Julia author Alvarez-Alegret, Ramiro author Ortiz-Duran, Rosa author Vicioso, Luis author Gomez-Dorronsoro, Maria Luisa author García Del Muro, Xavier author Martinez-Trufero, Javier author Rubio-Casadevall, Jordi author Sevilla, Isabel author Lainez, Nuria author Gutierrez, Antonio author Serrano, Cesar author Lopez-Alvarez, Maria author Hindi, Nadia author Taron, Miguel author Lopez-Guerrero, Jose Antonio author Martin-Broto, Javier author 2018 Introduction: There are limited findings available on KIT-negative GIST-like (KNGL) population. Also, KIT expression may be post-transcriptionally regulated by miRNA221 and miRNA222. Hence, the aim of this study is to characterize KNGL population, by differential gene expression, and to analyze miRNA221/222 expression and their prognostic value in KNGL patients.Methods: KIT, PDGFRA, DOG1, IGF1R, MIR221 and MIR222 expression levels were determined by qRT-PCR. We also analyzed KIT and PDGFRA mutations, DOG1 expression, by immunohistochemistry, along with clinical and pathological data. Disease-free survival (DFS) and overall survival (OS) differences were calculated using Log-rank test.Results: Hierarchical cluster analyses from gene expression data identified two groups: group I had KIT, DOG1 and PDGFRA overexpression and IGF1R underexpression and group II had overexpression of IGF1R and low expression of KIT, DOG1 and PDGFRA. Group II had a significant worse OS (p = 0.013) in all the series, and showed a tendency for worse OS (p = 0.11), when analyzed only the localized cases. MiRNA222 expression was significantly lower in a control subset of KIT-positive GIST (p < 0.001). OS was significantly worse in KNGL cases with higher expression of MIR221 (p = 0.028) or MIR222 (p = 0.014).Conclusions: We identified two distinct KNGL subsets, with a different prognostic value. Increased levels of miRNA221/222, which are associated with worse OS, could explain the absence of KIT protein expression of most KNGL tumors. Moura DS, Ramos R, Fernandez-Serra A, Serrano T, Cruz J, Alvarez-Alegret R, et al. Gene expression analyses determine two different subpopulations in KIT-negative GIST-like (KNGL) patients.. Oncotarget. 2018;9(25):17576-17588. 10.18632/oncotarget.24799 1949-2553 Oncotarget http://hdl.handle.net/20.500.13003/17091 29707131 L621545153 2-s2.0-85044833455 https://hdl.handle.net/20.500.12105/22622 DOG1 IGF1R KIT KNGL miRNA221/222 cluster Gene expression analyses determine two different subpopulations in KIT-negative GIST-like (KNGL) patients.