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oai:repisalud.isciii.es:20.500.12105/225932024-11-29T01:35:57Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967
Repisalud author Busquets-Cortés, Carla author Capó Fiol, Xavier author Argelich, Emma author Ferrer, Miguel D author Mateos, David author Bouzas, Cristina author Abbate, Manuela author Tur, Josep A author Soldevila Verdeguer, Carla author Pons, Antoni 2024-09-06T09:56:02Z 2024-09-06T09:56:02Z 2018-12 Busquets-Cortes C, Capo X, Argelich E, Ferrer MD, Mateos D, Bouzas C, et al. Effects of Millimolar Steady-State Hydrogen Peroxide Exposure on Inflammatory and Redox Gene Expression in Immune Cells from Humans with Metabolic Syndrome. Nutrients. 2018 Dec;10(12):1920. 10.3390/nu10121920 2072-6643 Nutrients http://hdl.handle.net/20.500.13003/8984 30563042 L625323307 2-s2.0-85058054679 https://hdl.handle.net/20.500.12105/22593 455073200101 Reactive oxygen species (ROS) such as hydrogen peroxide (H2O2) can exert opposed effects depending on the dosage: low levels can be involved in signalling and adaptive processes, while higher levels can exert deleterious effects in cells and tissues. Our aim was to emulate a chronic ex vivo oxidative stress situation through a 2 h exposure of immune cells to sustained H2O2 produced by glucose oxidase (GOX), at high or low production rate, in order to determine dissimilar responses of peripheral blood mononuclear cells (PBMCs) and neutrophils on ROS and cytokine production, and mitochondrial dynamics-related proteins, pro/anti-inflammatory and anti-oxidant gene expression. Immune cells were obtained from subjects with metabolic syndrome. H2O2 at low concentrations can trigger a transient anti-inflammatory adiponectin secretion and reduced gene expression of toll-like receptors (TLRs) in PBMCs but may act as a stimulator of proinflammatory genes (IL6, IL8) and mitochondrial dynamics-related proteins (Mtf2, NRF2, Tfam). H2O2 at a high concentration enhances the expression of pro-inflammatory genes (TLR2 and IL1) and diminishes the expression of mitochondrial dynamics-related proteins (Mtf1, Tfam) and antioxidant enzymes (Cu/Zn SOD) in PBMCs. The GOX treatments produce dissimilar changes in immune cells: Neutrophils were more resistant to H2O2 effects and exhibited a more constant response in terms of gene expression than PBMCs. We observe emerging roles of H2O2 in mitochondrial dynamics and redox and inflammation processes in immune cells. eng Gene expression Glucose oxidase Hydrogen peroxide Inflammation Mitochondrial biogenesis Neutrophils PBMCs ROS Effects of Millimolar Steady-State Hydrogen Peroxide Exposure on Inflammatory and Redox Gene Expression in Immune Cells from Humans with Metabolic Syndrome research article