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oai:repisalud.isciii.es:20.500.12105/225862024-11-28T21:31:48Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967

00925njm 22002777a 4500 dc Cordoba, Alba author Manzanaro-Moreno, Nahuel author Colom, Carme author Ronold, Hans J author Lyngstadaas, Staale P author Monjo, Marta author Ramis, Joana Maria author 2018-11 In this study, the effect on osteoclast activity in vitro and in vivo of titanium implants that were coated with quercitrin was evaluated. Titanium surfaces were covalently coated with the flavonoid quercitrin. The effect of the surfaces on osteoclastogenesis was first tested in vitro on RAW264.7 cells that were supplemented with receptor activator of nuclear factor kappa-B ligand (RANKL) to generate osteoclast-like cells by tartrate-resistant acid phosphatase (TRAP) inmunostaining after five days of culture, and by analysis of the mRNA expression levels of markers related to bone resorption after seven days of culture. A rabbit tibial model was used to evaluate the in vivo biological response to the implant surfaces after eight weeks of healing, analyzing the lactate dehydrogenase (LDH) and the alkaline phosphatase (ALP) activities in the wound fluid that were present at the implant interface and the peri-implant bone mRNA expression levels of several markers related to inflammation, bone resorption and osteoblast-osteoclast interaction. No differences between groups and control surfaces were found in the wound fluid analyses. Moreover, quercitrin implant surfaces significantly decreased the expression of osteoclast related genes in vitro (Trap, CalcR, Ctsk, H(+)ATPase, Mmp9) and in vivo (Ctsk, H(+)ATPase, Mmp9) as well as the expression of RankL in vivo. Moreover, quercitrin surfaces were not cytotoxic for the cells. Thus, quercitrin implant surfaces were biocompatible and decreased osteoclastogenesis in vitro and in vivo. This could be used to improve the performance of dental implants. Cordoba A, Manzanaro-Moreno N, Colom C, Ronold Hans J, Lyngstadaas Staale P, Monjo M, et al. Quercitrin Nanocoated Implant Surfaces Reduce Osteoclast Activity In Vitro and In Vivo. Int J Mol Sci. 2018 Nov;19(11):3319. 1422-0067 http://hdl.handle.net/20.500.13003/9037 https://hdl.handle.net/20.500.12105/22586 30366383 10.3390/ijms19113319 International Journal of Molecular Sciences 2-s2.0-85055613213 451528500035 L624753343 Bone implant interactions Surface chemistry Animal experiments Biomaterials Polyphenols Quercitrin Nanocoated Implant Surfaces Reduce Osteoclast Activity In Vitro and In Vivo