2026-04-29T02:37:50Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/225812024-11-29T03:40:46Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967
Repisalud
author
Ferrà-Cañellas, Maria Del Mar
author
Antonia Llopis-Grimalt, Maria
author
Monjo, Marta
author
Maria Ramis, Joana
2024-09-06T09:55:59Z
2024-09-06T09:55:59Z
2018-10
Ferrá Cañellas MM, Llopis Grimalt MA, Monjo M, Ramis JM. Tuning Nanopore Diameter of Titanium Surfaces to Improve Human Gingival Fibroblast Response. Int J Mol Sci. 2018 Oct;19(10):2881.
http://hdl.handle.net/20.500.13003/9106
https://hdl.handle.net/20.500.12105/22581
30249013
10.3390/ijms19102881
1422-0067
International Journal of Molecular Sciences
2-s2.0-85053851839
448951000036
L624002053
The aim of this study was to determine the optimal nanopore diameter of titanium nanostructured surfaces to improve human gingival fibroblast (hGF) response, with the purpose of promoting gingiva integration to dental implant abutments. Two TiO2 nanoporous groups with different diameters (NP-S 48 nm and NP-B 74 nm) were grown on Ti foils using an organic electrolyte containing fluoride by electrochemical oxidation, varying the applied voltage and the interelectrode spacing. The surfaces were characterized by scanning electron microscope (SEM), atomic force microscopy (AFM), and contact angle. The hGF were cultured onto the different surfaces, and metabolic activity, cytotoxicity, cell adhesion, and gene expression were analyzed. Bigger porous diameters (NP-B) were obtained by increasing the voltage used during anodization. To obtain the smallest diameter (NP-S), apart from lowering the voltage, a lower interelectrode spacing was needed. The greatest surface area and number of peaks was found for NP-B, despite these samples not being the roughest as defined by R-a. NP-B had a better cellular response compared to NP-S. However, these effects had a significant dependence on the cell donor. In conclusion, nanoporous groups with a diameter in the range of 74 nm induce a better hGF response, which may be beneficial for an effective soft tissue integration around the implant.
eng
Nanopore diameter
Electrochemical anodization
Soft tissue integration
Surface area
Tuning Nanopore Diameter of Titanium Surfaces to Improve Human Gingival Fibroblast Response
research article