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00925njm 22002777a 4500 dc Ferrà-Cañellas, Maria Del Mar author Antonia Llopis-Grimalt, Maria author Monjo, Marta author Maria Ramis, Joana author 2018-10 The aim of this study was to determine the optimal nanopore diameter of titanium nanostructured surfaces to improve human gingival fibroblast (hGF) response, with the purpose of promoting gingiva integration to dental implant abutments. Two TiO2 nanoporous groups with different diameters (NP-S 48 nm and NP-B 74 nm) were grown on Ti foils using an organic electrolyte containing fluoride by electrochemical oxidation, varying the applied voltage and the interelectrode spacing. The surfaces were characterized by scanning electron microscope (SEM), atomic force microscopy (AFM), and contact angle. The hGF were cultured onto the different surfaces, and metabolic activity, cytotoxicity, cell adhesion, and gene expression were analyzed. Bigger porous diameters (NP-B) were obtained by increasing the voltage used during anodization. To obtain the smallest diameter (NP-S), apart from lowering the voltage, a lower interelectrode spacing was needed. The greatest surface area and number of peaks was found for NP-B, despite these samples not being the roughest as defined by R-a. NP-B had a better cellular response compared to NP-S. However, these effects had a significant dependence on the cell donor. In conclusion, nanoporous groups with a diameter in the range of 74 nm induce a better hGF response, which may be beneficial for an effective soft tissue integration around the implant. Ferrá Cañellas MM, Llopis Grimalt MA, Monjo M, Ramis JM. Tuning Nanopore Diameter of Titanium Surfaces to Improve Human Gingival Fibroblast Response. Int J Mol Sci. 2018 Oct;19(10):2881. http://hdl.handle.net/20.500.13003/9106 https://hdl.handle.net/20.500.12105/22581 30249013 10.3390/ijms19102881 1422-0067 International Journal of Molecular Sciences 2-s2.0-85053851839 448951000036 L624002053 Nanopore diameter Electrochemical anodization Soft tissue integration Surface area Tuning Nanopore Diameter of Titanium Surfaces to Improve Human Gingival Fibroblast Response