2026-04-29T07:22:12Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/205212024-09-28T03:21:51Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617
Repisalud
author
Torrente-Rodríguez, Rebeca M
author
Ruiz-Valdepeñas Montiel, Víctor
author
Iftimie, Simona
author
Montero-Calle, Ana Maria
author
Pingarrón, José M
author
Castro, Antoni
author
Camps, Jordi
author
Barderas Manchado, Rodrigo
author
Campuzano, Susana
author
Joven, Jorge
funder
Consejo Superior de Investigaciones Científicas (España)
funder
Conferencia de Rectores de las Universidades Españolas
funder
Agencia Estatal de Investigación (España)
funder
Ministerio de Ciencia e Innovación (España)
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Fundación La Marató TV3
2024-07-12T07:20:09Z
2024-07-12T07:20:09Z
2024-06-04
Mikrochim Acta. 2024 Jun 4;191(7):369.
http://hdl.handle.net/20.500.12105/20521
38834823
10.1007/s00604-024-06440-y
1436-5073
Mikrochimica acta
A trendsetting direct competitive-based biosensing tool has been developed and implemented for the determination of the polyunsaturated fatty acid arachidonic acid (ARA), a highly significant biological regulator with decisive roles in viral infections. The designed methodology involves a competitive reaction between the target endogenous ARA and a biotin-ARA competitor for the recognition sites of anti-ARA antibodies covalently attached to the surface of carboxylic acid-coated magnetic microbeads (HOOC-MµBs), followed by the enzymatic label of the biotin-ARA residues with streptavidin-horseradish peroxidase (Strep-HRP) conjugate. The resulting bioconjugates were magnetically trapped onto the sensing surface of disposable screen-printed carbon transducers (SPCEs) to monitor the extent of the biorecognition reaction through amperometry. The operational functioning of the exhaustively optimized and characterized immunosensing bioplatform was highly convenient for the quantitative determination of ARA in serum samples from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2-) and respiratory syncytial virus (RSV)-infected individuals in a rapid, affordable, trustful, and sensitive manner.
eng
Electrochemical bioplatform
Amperometry
Screen-printed carbon electrode
Arachidonic acid
Serum samples
SARS-CoV-2
RSV
Contributing to the management of viral infections through simple immunosensing of the arachidonic acid serum level
research article
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ContributingManagementViralInfections_2024.pdf
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Supplementary_ContributingManagementViralInfections_2024.pdf
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ContributingManagementViralInfections_2024.pdf.txt
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