2026-04-29T07:22:53Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/205212024-09-28T03:21:51Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617

00925njm 22002777a 4500 dc Torrente-Rodríguez, Rebeca M author Ruiz-Valdepeñas Montiel, Víctor author Iftimie, Simona author Montero-Calle, Ana Maria author Pingarrón, José M author Castro, Antoni author Camps, Jordi author Barderas Manchado, Rodrigo author Campuzano, Susana author Joven, Jorge author 2024-06-04 A trendsetting direct competitive-based biosensing tool has been developed and implemented for the determination of the polyunsaturated fatty acid arachidonic acid (ARA), a highly significant biological regulator with decisive roles in viral infections. The designed methodology involves a competitive reaction between the target endogenous ARA and a biotin-ARA competitor for the recognition sites of anti-ARA antibodies covalently attached to the surface of carboxylic acid-coated magnetic microbeads (HOOC-MµBs), followed by the enzymatic label of the biotin-ARA residues with streptavidin-horseradish peroxidase (Strep-HRP) conjugate. The resulting bioconjugates were magnetically trapped onto the sensing surface of disposable screen-printed carbon transducers (SPCEs) to monitor the extent of the biorecognition reaction through amperometry. The operational functioning of the exhaustively optimized and characterized immunosensing bioplatform was highly convenient for the quantitative determination of ARA in serum samples from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2-) and respiratory syncytial virus (RSV)-infected individuals in a rapid, affordable, trustful, and sensitive manner. Mikrochim Acta. 2024 Jun 4;191(7):369. http://hdl.handle.net/20.500.12105/20521 38834823 10.1007/s00604-024-06440-y 1436-5073 Mikrochimica acta Electrochemical bioplatform Amperometry Screen-printed carbon electrode Arachidonic acid Serum samples SARS-CoV-2 RSV Contributing to the management of viral infections through simple immunosensing of the arachidonic acid serum level