2026-05-08T18:40:46Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/202782025-05-07T08:54:58Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Lozano-Chamizo, Laura
author
Márquez, Carlos
author
Marciello, Marzia
author
Galdon, José Carlos
author
de la Fuente-Zapico, Elsa
author
Martinez-Mazón, Paula
author
González-Rumayor, Víctor
author
Filice, Marco
author
Gamiz, Francisco
funder
Comunidad de Madrid (España)
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Ministerio de Ciencia e Innovación (España)
funder
Fundación ProCNIC
funder
Unión Europea. Comisión Europea. NextGenerationEU
funder
Agencia Estatal de Investigación (España)
2024-07-09T09:14:10Z
2024-07-09T09:14:10Z
2024-04-15
Biosens Bioelectron. 2024 Apr 15:250:116040.
10.1016/j.bios.2024.116040
1873-4235
Biosensors & bioelectronics
38290380
http://hdl.handle.net/20.500.12105/20278
The COVID-19 pandemic has taught us valuable lessons, especially the urgent need for a widespread, rapid and sensitive diagnostic tool. To this, the integration of bidimensional nanomaterials, particularly graphene, into point-of-care biomedical devices is a groundbreaking strategy able to potentially revolutionize the diagnostic landscape. Despite advancements in the fabrication of these biosensors, the relationship between their surface biofunctionalization and sensing performance remains unclear. Here, we demonstrate that the combination of careful sensor fabrication and its precise surface biofunctionalization is crucial for exalting the sensing performances of 2D biosensors. Specifically, we have biofunctionalized Graphene Field-Effect Transistor (GFET) sensors surface through different biochemical reactions to promote either random/heterogeneous or oriented/homogeneous immobilization of the Anti-SARS-CoV-2 spike protein antibody. Each strategy was thoroughly characterized by in-silico simulations, physicochemical and biochemical techniques and electrical characterization. Subsequently, both biosensors were tested in the label-free direct titration of SARS-CoV-2 virus in simulated clinical samples, avoiding sample preprocessing and within short timeframes. Remarkably, the oriented GFET biosensor exhibited significantly enhanced reproducibility and responsiveness, surpassing the detection sensitivity of conventional non-oriented GFET by more than twofold. This breakthrough not only involves direct implications for COVID-19 surveillance and next pandemic preparedness but also clarify an unexplored mechanistic dimension of biosensor research utilizing 2D-nanomaterials.
eng
High enhancement of sensitivity and reproducibility in label-free SARS-CoV-2 detection with graphene field-effect transistor sensors through precise surface biofunctionalization control.
journal article
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High enhancement of sensitivity_Biosens Bioelectron_2024.pdf
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https://repisalud.isciii.es/bitstreams/52b708e6-cf4b-4de6-a8e3-cbb0e76bfb30/download
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text/plain
High enhancement of sensitivity_Biosens Bioelectron_2024.pdf.txt