2026-05-08T18:40:32Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/202052024-09-27T07:55:32Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Garcia-Gonzalez, Irene
author
Rocha, Susana F
author
Hamidi, Anahita
author
Garcia-Ortega, Lourdes
author
Regano, Alvaro
author
Sanchez-Muñoz, Maria S
author
Lytvyn, Mariya
author
Garcia-Cabero, Aroa
author
Roig-Soucase, Sergi
author
Schoofs, Hans
author
Castro, Marco
author
Sabata, Helena
author
Potente, Michael
author
Graupera, Mariona
author
Makinen, Taija
author
Benedito, Rui
funder
Ministerio de Ciencia e Innovación (España)
funder
Instituto de Salud Carlos III
funder
Fundación La Caixa
funder
Unión Europea. Comisión Europea. European Research Council (ERC)
funder
Unión Europea. Comisión Europea. H2020
funder
Marie Curie
funder
Fundación ProCNIC
funder
Ministerio de Ciencia e Innovación. Centro de Excelencia Severo Ochoa (España)
2024-07-08T12:17:33Z
2024-07-08T12:17:33Z
2024-06-08
Nucleic Acids Res. 2024 Jun 8:gkae472.
10.1093/nar/gkae472
1362-4962
Nucleic acids research
38850155
http://hdl.handle.net/20.500.12105/20205
Methods for modifying gene function at high spatiotemporal resolution in mice have revolutionized biomedical research, with Cre-loxP being the most widely used technology. However, the Cre-loxP technology has several drawbacks, including weak activity, leakiness, toxicity, and low reliability of existing Cre-reporters. This is mainly because different genes flanked by loxP sites (floxed) vary widely in their sensitivity to Cre-mediated recombination. Here, we report the generation, validation, and utility of iSuRe-HadCre, a new dual Cre-reporter and deleter mouse line that avoids these drawbacks. iSuRe-HadCre achieves this through a novel inducible dual-recombinase genetic cascade that ensures that cells expressing a fluorescent reporter had only transient Cre activity, that is nonetheless sufficient to effectively delete floxed genes. iSuRe-HadCre worked reliably in all cell types and for the 13 floxed genes tested. This new tool will enable the precise, efficient, and trustworthy analysis of gene function in entire mouse tissues or in single cells.
eng
iSuRe-HadCre is an essential tool for effective conditional genetics.
journal article
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iSuRe-HadCre is an essential tool_Nucleic Acids Res_2024.pdf
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https://repisalud.isciii.es/bitstreams/b29fce3d-c1c9-49b2-8c1f-0b23c9cee6cf/download
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115970
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iSuRe-HadCre is an essential tool_Nucleic Acids Res_2024.pdf.txt