2026-06-14T03:41:06Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/201692024-11-28T21:48:41Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967
Repisalud
author
Petrov, Petar D.
author
Granados, Nuria
author
Chetrit, Carles
author
Martinez-Puig, Daniel
author
Palou, Andreu
author
Luisa Bonet, M.
2024-07-04T12:56:37Z
2024-07-04T12:56:37Z
2015
Petrov Petar D, Granados N, Chetrit C, Martinez-Puig D, Palou Oliver A, Bonet ML. Synergistic Effects of a Mixture of Glycosaminoglycans to Inhibit Adipogenesis and Enhance Chondrocyte Features in Multipotent Cells. Cell Physiol Biochem. 2015;37(5):1792-806. Epub 2015 Nov 13.
10.1159/000438542
1421-9778
1015-8987
Cellular Physiology and Biochemistry
http://hdl.handle.net/20.500.13003/11666
26584280
L607074983
2-s2.0-84948151169
http://hdl.handle.net/20.500.12105/20169
365604500013
Background/Aims: Multipotent mesenchymal stem cells affect homeostasis of adipose and joint tissues. Factors influencing their differentiation fate are of interest for both obesity and joint problems. We studied the impact of a mixture of glycosaminoglycans (GAGs) (hyaluronic acid: dermatan sulfate 1:0.25, w/w) used in an oral supplement for joint discomfort (OralviscTM) on the differentiation fate of multipotent cells. Methods: Primary mouse embryo fibroblasts (MEFs) were used as a model system. Post-confluent monolayer MEF cultures non-stimulated or hormonally stimulated to adipogenesis were chronically exposed to the GAGs mixture, its individual components or vehicle. The appearance of lipid laden cells, lipid accumulation and expression of selected genes at the mRNA and protein level was assessed. Results: Exposure to the GAGs mixture synergistically suppressed spontaneous adipogenesis and induced the expression of cartilage extracellular matrix proteins, aggrecan core protein, decorin and cartilage oligomeric matrix protein. Hormonally-induced adipogenesis in the presence of the GAGs mixture resulted in decreased ad ipogenic differentiation, down-regulation of ad ipogenic/ lipogenic factors and genes for insulin resistance-related adipokines (resistin and retinol binding protein 4), and up-regulation of oxidative metabolism-related genes. Adipogenesis in the presence of dermatan sulfate, the minor component of the mixture, was not impaired but resulted in smaller lipid droplets and the induction of a more complete brown adipocyterelated transcriptional program in the cells in the adipose state. Conclusions: The Oralviscm GAGs mixture can tip the adipogenic/chondrogenic fate balance of multipotent cells away from adipogenesis while favoring chondrocyte related gene expression. The mixture and its dermatan sulfate component also have modulatory effects of interest on hormonally-induced adipogenesis and on metabolic and secretory capabilities of adipose cells.
eng
Glycosaminoglycans
Adipogenesis
Chondrogenesis
Osteoarthritis
Joint health
Obesity
Synergistic Effects of a Mixture of Glycosaminoglycans to Inhibit Adipogenesis and Enhance Chondrocyte Features in Multipotent Cells
research article